RRC ID 45875
著者 Wang P, Liu B, Zhang D, Belew MY, Tissenbaum HA, Cheng JX.
タイトル Imaging lipid metabolism in live Caenorhabditis elegans using fingerprint vibrations.
ジャーナル Angew Chem Int Ed Engl
Abstract Quantitation of lipid storage, unsaturation, and oxidation in live C. elegans has been a long-standing obstacle. The combination of hyperspectral stimulated Raman scattering imaging and multivariate analysis in the fingerprint vibration region represents a platform that allows the quantitative mapping of fat distribution, degree of fat unsaturation, lipid oxidation, and cholesterol storage in vivo in the whole worm. Our results reveal for the first time that lysosome-related organelles in intestinal cells are sites for storage of cholesterol in C. elegans.
巻・号 53(44)
ページ 11787-92
公開日 2014-10-27
DOI 10.1002/anie.201406029
PMID 25195517
PMC PMC4348091
MeSH Animals Caenorhabditis elegans / chemistry* Dermatoglyphics Lipids / chemistry* Metabolic Networks and Pathways* Spectrum Analysis / methods* Vibration
IF 12.959
引用数 45
WOS 分野 CHEMISTRY, MULTIDISCIPLINARY
リソース情報
線虫 tm331 tm420