RRC ID 45826
著者 Patananan AN, Budenholzer LM, Pedraza ME, Torres ER, Adler LN, Clarke SG.
タイトル The invertebrate Caenorhabditis elegans biosynthesizes ascorbate.
ジャーナル Arch Biochem Biophys
Abstract l-Ascorbate, commonly known as vitamin C, serves as an antioxidant and cofactor essential for many biological processes. Distinct ascorbate biosynthetic pathways have been established for animals and plants, but little is known about the presence or synthesis of this molecule in invertebrate species. We have investigated ascorbate metabolism in the nematode Caenorhabditis elegans, where this molecule would be expected to play roles in oxidative stress resistance and as cofactor in collagen and neurotransmitter synthesis. Using high-performance liquid chromatography and gas-chromatography mass spectrometry, we determined that ascorbate is present at low amounts in the egg stage, L1 larvae, and mixed animal populations, with the egg stage containing the highest concentrations. Incubating C. elegans with precursor molecules necessary for ascorbate synthesis in plants and animals did not significantly alter ascorbate levels. Furthermore, bioinformatic analyses did not support the presence in C. elegans of either the plant or the animal biosynthetic pathway. However, we observed the complete (13)C-labeling of ascorbate when C. elegans was grown with (13)C-labeled Escherichia coli as a food source. These results support the hypothesis that ascorbate biosynthesis in invertebrates may proceed by a novel pathway and lay the foundation for a broader understanding of its biological role.
巻・号 569
ページ 32-44
公開日 2015-3-1
DOI 10.1016/j.abb.2015.02.002
PII S0003-9861(15)00055-7
PMID 25668719
PMC PMC4357563
MeSH Animals Antioxidants / metabolism Ascorbic Acid / biosynthesis* Caenorhabditis elegans / genetics Caenorhabditis elegans / growth & development Caenorhabditis elegans / metabolism* Carbon Isotopes Chromatography, High Pressure Liquid Ethanol / toxicity Gas Chromatography-Mass Spectrometry Genes, Helminth Metabolic Networks and Pathways Oxidative Stress / drug effects Paraquat / toxicity
IF 3.391
引用数 7
WOS 分野 BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
線虫 tm2679