RRC ID 45771
著者 Kim Y, Rosenberg SC, Kugel CL, Kostow N, Rog O, Davydov V, Su TY, Dernburg AF, Corbett KD.
タイトル The chromosome axis controls meiotic events through a hierarchical assembly of HORMA domain proteins.
ジャーナル Dev Cell
Abstract Proteins of the HORMA domain family play central, but poorly understood, roles in chromosome organization and dynamics during meiosis. In Caenorhabditis elegans, four such proteins (HIM-3, HTP-1, HTP-2, and HTP-3) have distinct but overlapping functions. Through combined biochemical, structural, and in vivo analysis, we find that these proteins form hierarchical complexes through binding of their HORMA domains to cognate peptides within their partners' C-terminal tails, analogous to the "safety belt" binding mechanism of Mad2. These interactions are critical for recruitment of HIM-3, HTP-1, and HTP-2 to chromosome axes. HTP-3, in addition to recruiting the other HORMA domain proteins to the axis, plays an independent role in sister chromatid cohesion and double-strand break formation. Finally, we find that mammalian HORMAD1 binds a motif found both at its own C terminus and at that of HORMAD2, indicating that this mode of intermolecular association is a conserved feature of meiotic chromosome structure in eukaryotes.
巻・号 31(4)
ページ 487-502
公開日 2014-11-24
DOI 10.1016/j.devcel.2014.09.013
PII S1534-5807(14)00619-4
PMID 25446517
PMC PMC4254552
MeSH Animals Caenorhabditis elegans / cytology Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / metabolism* Cell Cycle Proteins / metabolism* Chromosome Pairing / genetics* Chromosome Segregation / physiology Chromosomes / metabolism* Meiosis / physiology* Mutation / genetics Synaptonemal Complex / metabolism*
IF 10.092
引用数 40
WOS 分野 DEVELOPMENTAL BIOLOGY CELL BIOLOGY
リソース情報
線虫 tm3655