RRC ID 45733
著者 Goh WS, Seah JW, Harrison EJ, Chen C, Hammell CM, Hannon GJ.
タイトル A genome-wide RNAi screen identifies factors required for distinct stages of C. elegans piRNA biogenesis.
ジャーナル Genes Dev
Abstract In animals, piRNAs and their associated Piwi proteins guard germ cell genomes against mobile genetic elements via an RNAi-like mechanism. In Caenorhabditis elegans, 21U-RNAs comprise the piRNA class, and these collaborate with 22G RNAs via unclear mechanisms to discriminate self from nonself and selectively and heritably silence the latter. Recent work indicates that 21U-RNAs are post-transcriptional processing products of individual transcription units that produce ∼ 26-nucleotide capped precursors. However, nothing is known of how the expression of precursors is controlled or how primary transcripts give rise to mature small RNAs. We conducted a genome-wide RNAi screen to identify components of the 21U biogenesis machinery. Screening by direct, quantitative PCR (qPCR)-based measurements of mature 21U-RNA levels, we identified 22 genes important for 21U-RNA production, termed TOFUs (Twenty-One-u Fouled Ups). We also identified seven genes that normally repress 21U production. By measuring mature 21U-RNA and precursor levels for the seven strongest hits from the screen, we assigned factors to discrete stages of 21U-RNA production. Our work identifies for the first time factors separately required for the transcription of 21U precursors and the processing of these precursors into mature 21U-RNAs, thereby providing a resource for studying the biogenesis of this important small RNA class.
巻・号 28(7)
ページ 797-807
公開日 2014-4-1
DOI 10.1101/gad.235622.113
PII 28/7/797
PMID 24696458
PMC PMC4015493
MeSH Animals Caenorhabditis elegans / genetics* Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism* Gene Expression Regulation, Developmental* Genome, Helminth / genetics* RNA Interference RNA, Small Interfering / biosynthesis* RNA, Small Interfering / genetics Reproducibility of Results
IF 9.527
引用数 16
WOS 分野 GENETICS & HEREDITY DEVELOPMENTAL BIOLOGY CELL BIOLOGY
リソース情報
線虫 tm6424 tm6145 tm872 tm3068 tm3071 tm3063 tm6157 tm892