RRC ID 45673
著者 Ailion M, Hannemann M, Dalton S, Pappas A, Watanabe S, Hegermann J, Liu Q, Han HF, Gu M, Goulding MQ, Sasidharan N, Schuske K, Hullett P, Eimer S, Jorgensen EM.
タイトル Two Rab2 interactors regulate dense-core vesicle maturation.
ジャーナル Neuron
Abstract Peptide neuromodulators are released from a unique organelle: the dense-core vesicle. Dense-core vesicles are generated at the trans-Golgi and then sort cargo during maturation before being secreted. To identify proteins that act in this pathway, we performed a genetic screen in Caenorhabditis elegans for mutants defective in dense-core vesicle function. We identified two conserved Rab2-binding proteins: RUND-1, a RUN domain protein, and CCCP-1, a coiled-coil protein. RUND-1 and CCCP-1 colocalize with RAB-2 at the Golgi, and rab-2, rund-1, and cccp-1 mutants have similar defects in sorting soluble and transmembrane dense-core vesicle cargos. RUND-1 also interacts with the Rab2 GAP protein TBC-8 and the BAR domain protein RIC-19, a RAB-2 effector. In summary, a pathway of conserved proteins controls the maturation of dense-core vesicles at the trans-Golgi network.
巻・号 82(1)
ページ 167-80
公開日 2014-4-2
DOI 10.1016/j.neuron.2014.02.017
PII S0896-6273(14)00115-9
PMID 24698274
PMC PMC3997996
MeSH Analysis of Variance Animals Animals, Genetically Modified Caenorhabditis elegans Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Chromosome Mapping Cloning, Molecular Endosomes / metabolism Endosomes / ultrastructure Golgi Apparatus / metabolism Golgi Apparatus / ultrastructure Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Microscopy, Electron, Transmission Molecular Sequence Data Mutation / genetics* Protein Transport Secretory Vesicles / genetics Secretory Vesicles / metabolism* Secretory Vesicles / ultrastructure rab2 GTP-Binding Protein / genetics rab2 GTP-Binding Protein / metabolism*
IF 14.415
引用数 30
WOS 分野 NEUROSCIENCES
リソース情報
線虫 tm3622