論文 - 詳細
| RRC ID | 45637 |
|---|---|
| 著者 | Sailer A, Anneken A, Li Y, Lee S, Munro E. |
| タイトル | Dynamic Opposition of Clustered Proteins Stabilizes Cortical Polarity in the C. elegans Zygote. |
| ジャーナル | Dev Cell |
| Abstract |
Dynamic maintenance of cell polarity is essential for development and physiology. Here we combine experiments and modeling to elucidate mechanisms that maintain cortical polarity in the C. elegans zygote. We show that polarity is dynamically stabilized by two coupled cross-inhibitory feedback loops: one involves the oligomeric scaffold PAR-3 and the kinase PAR-1, and the other involves CDC-42 and its putative GAP CHIN-1. PAR-3 and CDC-42 are both required locally to recruit PAR-6/PKC-3, which inhibits PAR-1 (shown previously) and inhibits local growth/accumulation of CHIN-1 clusters. Conversely, PAR-1 inhibits local accumulation of PAR-3 oligomers, while CHIN-1 inhibits CDC-42 (shown previously), such that either PAR-1 or CHIN-1 can prevent recruitment of PAR-6/PKC-3, but loss of both causes complete loss of polarity. Ultrasensitive dependence of CHIN-1 cluster growth on PAR-6/PKC-3 endows this core circuit with bistable dynamics, while transport of CHIN-1 clusters by cortical flow can stabilize the AP boundary against diffusive spread of PAR-6/PKC-3. |
| 巻・号 | 35(1) |
| ページ | 131-42 |
| 公開日 | 2015-10-12 |
| DOI | 10.1016/j.devcel.2015.09.006 |
| PII | S1534-5807(15)00586-9 |
| PMID | 26460948 |
| PMC | PMC5963695 |
| MeSH | Animals Caenorhabditis elegans / cytology* Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism Cell Polarity* Embryo, Nonmammalian / cytology* Embryo, Nonmammalian / metabolism GTP-Binding Proteins / genetics GTP-Binding Proteins / metabolism GTPase-Activating Proteins / genetics GTPase-Activating Proteins / metabolism Gene Expression Regulation, Developmental* Image Processing, Computer-Assisted Protein Kinase C / genetics Protein Kinase C / metabolism Protein Serine-Threonine Kinases / genetics Protein Serine-Threonine Kinases / metabolism Zygote / cytology* Zygote / metabolism |
| IF | 10.092 |
| 引用数 | 30 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY CELL BIOLOGY |
| リソース情報 | |
| 線虫 | tm2616 tm1909 |