RRC ID 45533
著者 Butuči M, Williams AB, Wong MM, Kramer B, Michael WM.
タイトル Zygotic Genome Activation Triggers Chromosome Damage and Checkpoint Signaling in C. elegans Primordial Germ Cells.
ジャーナル Dev Cell
Abstract Recent findings have identified highly transcribed genes as a source of genome instability; however, the degree to which large-scale shifts in transcriptional activity cause DNA damage was not known. One example of a large-scale shift in transcriptional activity occurs during development, when maternal regulators are destroyed and zygotic genome activation (ZGA) occurs. Here, we show that ZGA triggers widespread chromosome damage in the primordial germ cells of the nematode C. elegans. We show that ZGA-induced DNA damage activates a checkpoint response, the damage is repaired by factors required for inter-sister homologous recombination, and topoisomerase II plays a role in generating the damage. These findings identify ZGA as a source of intrinsic genome instability in the germline and suggest that genome destabilization may be a general consequence of extreme shifts in cellular transcriptional load.
巻・号 34(1)
ページ 85-95
公開日 2015-7-6
DOI 10.1016/j.devcel.2015.04.019
PII S1534-5807(15)00283-X
PMID 26073019
MeSH Animals Caenorhabditis elegans / cytology* Caenorhabditis elegans / genetics Caenorhabditis elegans Proteins / genetics* Cell Cycle Checkpoints / genetics* DNA Damage / genetics* Genomic Instability / genetics* Germ Cells / cytology* Mutation / genetics Zygote / cytology*
IF 10.092
引用数 16
WOS 分野 DEVELOPMENTAL BIOLOGY CELL BIOLOGY
リソース情報
線虫 tm1145