| RRC ID |
4466
|
| 著者 |
Rocheleau CE, Cullison K, Huang K, Bernstein Y, Spilker AC, Sundaram MV.
|
| タイトル |
The Caenorhabditis elegans ekl (enhancer of ksr-1 lethality) genes include putative components of a germline small RNA pathway.
|
| ジャーナル |
Genetics
|
| Abstract |
A canonical Ras-ERK signaling pathway specifies the fate of the excretory duct cell during Caenorhabditis elegans embryogenesis. The paralogs ksr-1 and ksr-2 encode scaffolding proteins that facilitate signaling through this pathway and that act redundantly to promote the excretory duct fate. In a genomewide RNAi screen for genes that, like ksr-2, are required in combination with ksr-1 for the excretory duct cell fate, we identified 16 "ekl" (enhancer of ksr-1 lethality) genes that are largely maternally required and that have molecular identities suggesting roles in transcriptional or post-transcriptional gene regulation. These include the Argonaute gene csr-1 and a specific subset of other genes implicated in endogenous small RNA processes, orthologs of multiple components of the NuA4/Tip60 histone acetyltransferase and CCR4/NOT deadenylase complexes, and conserved enzymes involved in ubiquitination and deubiquitination. The identification of four small RNA regulators (csr-1, drh-3, ego-1, and ekl-1) that share the Ekl phenotype suggests that these genes define a functional pathway required for the production and/or function of particular germline small RNA(s). These small RNAs and the other ekl genes likely control the expression of one or more regulators of Ras-ERK signaling that function at or near the level of kinase suppressor of Ras (KSR).
|
| 巻・号 |
178(3)
|
| ページ |
1431-43
|
| 公開日 |
2008-3-1
|
| DOI |
10.1534/genetics.107.084608
|
| PII |
genetics.107.084608
|
| PMID |
18245826
|
| PMC |
PMC2278074
|
| MeSH |
Animals
Caenorhabditis elegans / cytology
Caenorhabditis elegans / enzymology
Caenorhabditis elegans / genetics*
Caenorhabditis elegans Proteins / genetics*
Conserved Sequence
Epistasis, Genetic
Gene Deletion
Genes, Helminth*
Germ Cells / metabolism*
Histone Acetyltransferases / metabolism
Larva
Models, Genetic
Mutation / genetics
Protein Kinases / metabolism
RNA Interference
RNA, Helminth / genetics*
RNA-Induced Silencing Complex
Ubiquitination
ras Proteins / metabolism
|
| IF |
4.015
|
| 引用数 |
34
|
|
WOS 分野
|
GENETICS & HEREDITY
|
| リソース情報 |
| 線虫 |
tm1531
tm1312
tm2531 |
