RRC ID 4320
著者 Yeh E, Ng S, Zhang M, Bouhours M, Wang Y, Wang M, Hung W, Aoyagi K, Melnik-Martinez K, Li M, Liu F, Schafer WR, Zhen M.
タイトル A putative cation channel, NCA-1, and a novel protein, UNC-80, transmit neuronal activity in C. elegans.
ジャーナル PLoS Biol
Abstract Voltage-gated cation channels regulate neuronal excitability through selective ion flux. NALCN, a member of a protein family that is structurally related to the alpha1 subunits of voltage-gated sodium/calcium channels, was recently shown to regulate the resting membrane potentials by mediating sodium leak and the firing of mouse neurons. We identified a role for the Caenorhabditis elegans NALCN homologues NCA-1 and NCA-2 in the propagation of neuronal activity from cell bodies to synapses. Loss of NCA activities leads to reduced synaptic transmission at neuromuscular junctions and frequent halting in locomotion. In vivo calcium imaging experiments further indicate that while calcium influx in the cell bodies of egg-laying motorneurons is unaffected by altered NCA activity, synaptic calcium transients are significantly reduced in nca loss-of-function mutants and increased in nca gain-of-function mutants. NCA-1 localizes along axons and is enriched at nonsynaptic regions. Its localization and function depend on UNC-79, and UNC-80, a novel conserved protein that is also enriched at nonsynaptic regions. We propose that NCA-1 and UNC-80 regulate neuronal activity at least in part by transmitting depolarization signals to synapses in C. elegans neurons.
巻・号 6(3)
ページ e55
公開日 2008-3-11
DOI 10.1371/journal.pbio.0060055
PII 07-PLBI-RA-2885
PMID 18336069
PMC PMC2265767
MeSH Animals Animals, Genetically Modified Caenorhabditis elegans / genetics* Caenorhabditis elegans / physiology Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism Caenorhabditis elegans Proteins / physiology* Cations / metabolism Cells, Cultured Cholinergic Fibers / metabolism Humans Ion Channels / genetics Ion Channels / metabolism Ion Channels / physiology* Models, Biological Nerve Tissue Proteins / genetics Nerve Tissue Proteins / metabolism Nerve Tissue Proteins / physiology* Neuromuscular Junction / metabolism Neurons / metabolism Neurons / physiology* Serotonin / metabolism Synaptic Transmission / genetics* Tissue Distribution Transfection gamma-Aminobutyric Acid / metabolism
IF 7.076
引用数 68
WOS 分野 BIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
線虫 tm1591