RRC ID |
4320
|
著者 |
Yeh E, Ng S, Zhang M, Bouhours M, Wang Y, Wang M, Hung W, Aoyagi K, Melnik-Martinez K, Li M, Liu F, Schafer WR, Zhen M.
|
タイトル |
A putative cation channel, NCA-1, and a novel protein, UNC-80, transmit neuronal activity in C. elegans.
|
ジャーナル |
PLoS Biol
|
Abstract |
Voltage-gated cation channels regulate neuronal excitability through selective ion flux. NALCN, a member of a protein family that is structurally related to the alpha1 subunits of voltage-gated sodium/calcium channels, was recently shown to regulate the resting membrane potentials by mediating sodium leak and the firing of mouse neurons. We identified a role for the Caenorhabditis elegans NALCN homologues NCA-1 and NCA-2 in the propagation of neuronal activity from cell bodies to synapses. Loss of NCA activities leads to reduced synaptic transmission at neuromuscular junctions and frequent halting in locomotion. In vivo calcium imaging experiments further indicate that while calcium influx in the cell bodies of egg-laying motorneurons is unaffected by altered NCA activity, synaptic calcium transients are significantly reduced in nca loss-of-function mutants and increased in nca gain-of-function mutants. NCA-1 localizes along axons and is enriched at nonsynaptic regions. Its localization and function depend on UNC-79, and UNC-80, a novel conserved protein that is also enriched at nonsynaptic regions. We propose that NCA-1 and UNC-80 regulate neuronal activity at least in part by transmitting depolarization signals to synapses in C. elegans neurons.
|
巻・号 |
6(3)
|
ページ |
e55
|
公開日 |
2008-3-11
|
DOI |
10.1371/journal.pbio.0060055
|
PII |
07-PLBI-RA-2885
|
PMID |
18336069
|
PMC |
PMC2265767
|
MeSH |
Animals
Animals, Genetically Modified
Caenorhabditis elegans / genetics*
Caenorhabditis elegans / physiology
Caenorhabditis elegans Proteins / genetics
Caenorhabditis elegans Proteins / metabolism
Caenorhabditis elegans Proteins / physiology*
Cations / metabolism
Cells, Cultured
Cholinergic Fibers / metabolism
Humans
Ion Channels / genetics
Ion Channels / metabolism
Ion Channels / physiology*
Models, Biological
Nerve Tissue Proteins / genetics
Nerve Tissue Proteins / metabolism
Nerve Tissue Proteins / physiology*
Neuromuscular Junction / metabolism
Neurons / metabolism
Neurons / physiology*
Serotonin / metabolism
Synaptic Transmission / genetics*
Tissue Distribution
Transfection
gamma-Aminobutyric Acid / metabolism
|
IF |
7.076
|
引用数 |
68
|
WOS 分野
|
BIOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
線虫 |
tm1591 |