RRC ID 4269
著者 Muzzini DM, Plevani P, Boulton SJ, Cassata G, Marini F.
タイトル Caenorhabditis elegans POLQ-1 and HEL-308 function in two distinct DNA interstrand cross-link repair pathways.
ジャーナル DNA Repair (Amst)
Abstract DNA interstrand cross-links (ICLs) are highly cytotoxic DNA lesions hindering DNA replication and transcription. Whereas in bacteria and yeast the molecular mechanisms involved in ICL repair are genetically well dissected, the scenario in multicellular organisms remains unclear. Here, we report that the two new mus308 genes, polq-1 and hel-308 are involved in ICL repair in Caenorhabditis elegans. After treatment with ICL agents, a decrease in survival and an increase in checkpoint-induced cell-cycle arrest and apoptosis of germ cells is observed in mutants of both genes. Although sensitive to ICL agents and to a minor extent to IR, cytological and epistatic analyses suggest that polq-1 and hel-308 are involved in different DNA repair pathways. While hel-308 functions in a Fanconi anemia-dependent pathway, polq-1 has a role in a novel distinct and brc-1 (CeBRCA1)-dependent ICL repair process in metazoans.
巻・号 7(6)
ページ 941-50
公開日 2008-6-1
DOI 10.1016/j.dnarep.2008.03.021
PII S1568-7864(08)00126-2
PMID 18472307
MeSH Animals Base Sequence Caenorhabditis elegans / enzymology* Caenorhabditis elegans / genetics DNA Primers DNA Repair* DNA-Directed DNA Polymerase / metabolism* Polymerase Chain Reaction RNA Interference
IF 3.339
引用数 60
WOS 分野 TOXICOLOGY GENETICS & HEREDITY
リソース情報
線虫 tm2026 tm2171 tm2134 tm1145 tm1298 tm2134