論文 - 詳細
| RRC ID | 426 |
|---|---|
| 著者 | Göbel V, Barrett PL, Hall DH, Fleming JT. |
| タイトル | Lumen morphogenesis in C. elegans requires the membrane-cytoskeleton linker erm-1. |
| ジャーナル | Dev Cell |
| Abstract |
Epithelial tubes are basic building blocks of complex organs, but their architectural requirements are not well understood. Here we show that erm-1 is a unique C. elegans ortholog of the ERM family of cytoskeleton-membrane linkers, with an essential role in lumen morphogenesis. ERM-1 localizes to the luminal membranes of those tubular organ epithelia which lack stabilization by cuticle. RNA interference (RNAi), a germline deletion, and overexpression of erm-1 cause cystic luminal phenotypes in these epithelia. Confocal and ultrastructural analyses indicate that erm-1 functions directly in apical membrane morphogenesis, rather than in epithelial polarity and junction assembly as has been previously proposed for ERMs. We also show that act-5/cytoplasmic actin and sma-1/beta-H-spectrin are required for lumen formation and functionally interact with erm-1. Our findings suggest that there are common structural constraints on the architecture of diverse organ lumina. |
| 巻・号 | 6(6) |
| ページ | 865-73 |
| 公開日 | 2004-6-1 |
| DOI | 10.1016/j.devcel.2004.05.018 |
| PII | S1534-5807(04)00171-6 |
| PMID | 15177034 |
| MeSH | Animals Body Patterning / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans / ultrastructure Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / isolation & purification Caenorhabditis elegans Proteins / metabolism* Cell Membrane / metabolism* Cell Membrane / ultrastructure Cell Polarity / genetics Cytoskeletal Proteins / genetics Cytoskeletal Proteins / isolation & purification Cytoskeletal Proteins / metabolism* Cytoskeleton / metabolism* Cytoskeleton / ultrastructure Drosophila Proteins / genetics Drosophila Proteins / metabolism Epithelial Cells / metabolism Epithelial Cells / ultrastructure Microscopy, Electron Organogenesis / genetics RNA Interference Saccharomyces cerevisiae Proteins / genetics Saccharomyces cerevisiae Proteins / metabolism Spectrin / genetics Spectrin / metabolism |
| IF | 10.092 |
| 引用数 | 104 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY CELL BIOLOGY |
| リソース情報 | |
| 線虫 | tm677 |