RRC ID |
4220
|
著者 |
de Carvalho CE, Zaaijer S, Smolikov S, Gu Y, Schumacher JM, Colaiácovo MP.
|
タイトル |
LAB-1 antagonizes the Aurora B kinase in C. elegans.
|
ジャーナル |
Genes Dev
|
Abstract |
The Shugoshin/Aurora circuitry that controls the timely release of cohesins from sister chromatids in meiosis and mitosis is widely conserved among eukaryotes, although little is known about its function in organisms whose chromosomes lack a localized centromere. Here we show that Caenorhabditis elegans chromosomes rely on an alternative mechanism to protect meiotic cohesin that is shugoshin-independent and instead involves the activity of a new chromosome-associated protein named LAB-1 (Long Arm of the Bivalent). LAB-1 preserves meiotic sister chromatid cohesion by restricting the localization of the C. elegans Aurora B kinase, AIR-2, to the interface between homologs via the activity of the PP1/Glc7 phosphatase GSP-2. The localization of LAB-1 to chromosomes of dividing embryos and the suppression of mitotic-specific defects in air-2 mutant embryos with reduced LAB-1 activity support a global role of LAB-1 in antagonizing AIR-2 in both meiosis and mitosis. Although the localization of a GFP fusion and the analysis of mutants and RNAi-mediated knockdowns downplay a role for the C. elegans shugoshin protein in cohesin protection, shugoshin nevertheless helps to ensure the high fidelity of chromosome segregation at metaphase I. We propose that, in C. elegans, a LAB-1-mediated mechanism evolved to offset the challenges of providing protection against separase activity throughout a larger chromosome area.
|
巻・号 |
22(20)
|
ページ |
2869-85
|
公開日 |
2008-10-15
|
DOI |
10.1101/gad.1691208
|
PII |
22/20/2869
|
PMID |
18923084
|
PMC |
PMC2569883
|
MeSH |
Adenosine Triphosphatases / metabolism
Amino Acid Sequence
Animals
Aurora Kinase B
Aurora Kinases
Caenorhabditis elegans
Caenorhabditis elegans Proteins / antagonists & inhibitors
Caenorhabditis elegans Proteins / genetics
Caenorhabditis elegans Proteins / metabolism*
Cell Cycle Proteins / metabolism
Chromatids / genetics
Chromatids / metabolism
Chromosomal Proteins, Non-Histone / antagonists & inhibitors
Chromosomal Proteins, Non-Histone / genetics
Chromosomal Proteins, Non-Histone / metabolism*
Chromosome Segregation
DNA-Binding Proteins / metabolism
Embryo, Nonmammalian / cytology
Embryo, Nonmammalian / metabolism
Fluorescent Antibody Technique
Gene Expression Regulation, Developmental*
Immunoglobulin G / immunology
Meiosis / physiology
Meiotic Prophase I / physiology
Mitosis / physiology
Molecular Sequence Data
Multiprotein Complexes / metabolism
Protein Serine-Threonine Kinases / antagonists & inhibitors*
Protein Serine-Threonine Kinases / genetics
Protein Serine-Threonine Kinases / metabolism
RNA, Helminth / genetics
RNA, Helminth / metabolism
RNA, Messenger / genetics
RNA, Messenger / metabolism
RNA, Small Interfering / pharmacology
Reverse Transcriptase Polymerase Chain Reaction
Sequence Homology, Amino Acid
Sister Chromatid Exchange
|
IF |
9.527
|
引用数 |
61
|
WOS 分野
|
GENETICS & HEREDITY
DEVELOPMENTAL BIOLOGY
CELL BIOLOGY
|
リソース情報 |
線虫 |
tm1791
tm2443
tm2344
tm301
tm3002 |