RRC ID 4219
著者 Das PP, Bagijn MP, Goldstein LD, Woolford JR, Lehrbach NJ, Sapetschnig A, Buhecha HR, Gilchrist MJ, Howe KL, Stark R, Matthews N, Berezikov E, Ketting RF, Tavaré S, Miska EA.
タイトル Piwi and piRNAs act upstream of an endogenous siRNA pathway to suppress Tc3 transposon mobility in the Caenorhabditis elegans germline.
ジャーナル Mol Cell
Abstract The Piwi proteins of the Argonaute superfamily are required for normal germline development in Drosophila, zebrafish, and mice and associate with 24-30 nucleotide RNAs termed piRNAs. We identify a class of 21 nucleotide RNAs, previously named 21U-RNAs, as the piRNAs of C. elegans. Piwi and piRNA expression is restricted to the male and female germline and independent of many proteins in other small-RNA pathways, including DCR-1. We show that Piwi is specifically required to silence Tc3, but not other Tc/mariner DNA transposons. Tc3 excision rates in the germline are increased at least 100-fold in piwi mutants as compared to wild-type. We find no evidence for a Ping-Pong model for piRNA amplification in C. elegans. Instead, we demonstrate that Piwi acts upstream of an endogenous siRNA pathway in Tc3 silencing. These data might suggest a link between piRNA and siRNA function.
巻・号 31(1)
ページ 79-90
公開日 2008-7-11
DOI 10.1016/j.molcel.2008.06.003
PII S1097-2765(08)00392-4
PMID 18571451
PMC PMC3353317
MeSH Animals Argonaute Proteins Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / metabolism* DNA Transposable Elements / genetics* Drosophila Proteins Female Gene Silencing Genes, Helminth Germ Cells / growth & development Germ Cells / metabolism* Male Proteins / genetics Proteins / metabolism* RNA, Helminth / metabolism RNA, Small Interfering / metabolism* RNA-Induced Silencing Complex Transposases / metabolism
IF 15.584
引用数 226
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
線虫 tm872