RRC ID 4218
著者 Cui M, Allen MA, Larsen A, Macmorris M, Han M, Blumenthal T.
タイトル Genes involved in pre-mRNA 3'-end formation and transcription termination revealed by a lin-15 operon Muv suppressor screen.
ジャーナル Proc Natl Acad Sci U S A
Abstract RNA polymerase II (Pol II) transcription termination involves two linked processes: mRNA 3'-end formation and release of Pol II from DNA. Signals for 3' processing are recognized by a protein complex that includes cleavage polyadenylation specificity factor (CPSF) and cleavage stimulation factor (CstF). Here we identify suppressors encoding proteins that play roles in processes at the 3' ends of genes by exploiting a mutation in which the 3' end of another gene is transposed into the first gene of the Caenorhabditis elegans lin-15 operon. As expected, genes encoding CPSF and CstF were identified in the screen. We also report three suppressors encoding proteins containing a domain that interacts with the C-terminal domain of Pol II (CID). We show that two of the CID proteins are needed for efficient 3' cleavage and thus may connect transcription termination with RNA cleavage. Furthermore, our results implicate a serine/arginine-rich (SR) protein, SRp20, in events following 3'-end cleavage, leading to termination of transcription.
巻・号 105(43)
ページ 16665-70
公開日 2008-10-28
DOI 10.1073/pnas.0807104105
PII 0807104105
PMID 18946043
PMC PMC2571909
MeSH Animals Caenorhabditis elegans / genetics* Caenorhabditis elegans Proteins* Cleavage And Polyadenylation Specificity Factor / genetics Cleavage And Polyadenylation Specificity Factor / metabolism Cleavage Stimulation Factor / genetics Cleavage Stimulation Factor / metabolism Operon RNA 3' End Processing* RNA Polymerase II / metabolism RNA Precursors / genetics* RNA-Binding Proteins Terminator Regions, Genetic Transcription Factors* Transcription, Genetic*
IF 9.412
引用数 38
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
線虫 tm2715 tm2802 tm367