| RRC ID |
418
|
| 著者 |
Sassa T, Ueda-Ohba H, Kitamura K, Harada S, Hosono R.
|
| タイトル |
Role of Caenorhabditis elegans protein phosphatase type 1, CeGLC-7 beta, in metaphase to anaphase transition during embryonic development.
|
| ジャーナル |
Exp Cell Res
|
| Abstract |
In Caenorhabditis elegans embryogenesis, phosphorylation events are critical to chromosomal changes. To investigate the dephosphorylation of chromosome behavior, we cloned and characterized the cDNA that encodes C. elegans protein phosphatase type 1 (CeGLC-7 beta), which is composed of 333 amino acids. CeGLC-7 beta possesses a highly conserved amino acid sequence with mammalian and Drosophila protein phosphatase 1. Here, we report on the contribution of CeGLC-7 beta to the dephosphorylation of histone H3 at anaphase. At the embryonic stage, CeGLC-7 beta is associated with the nuclear membrane and chromosomes. The deletion of the Ceglc-7 beta gene and a microinjection of double-stranded RNA produce a disorganized embryogenesis. The Ceglc-7 beta gene mutation causes an abnormal accumulation of phosphorylated histone H3 and delays the mitotic process after anaphase. We propose that CeGLC-7 beta is involved in chromosome dynamics including histone H3 dephosphorylation.
|
| 巻・号 |
287(2)
|
| ページ |
350-60
|
| 公開日 |
2003-7-15
|
| DOI |
10.1016/s0014-4827(03)00157-5
|
| PII |
S0014482703001575
|
| PMID |
12837290
|
| MeSH |
Amino Acid Sequence
Anaphase / genetics
Animals
Base Sequence
Caenorhabditis elegans / embryology
Caenorhabditis elegans / enzymology*
Caenorhabditis elegans / genetics
Caenorhabditis elegans Proteins / genetics
Caenorhabditis elegans Proteins / metabolism*
Conserved Sequence
Embryo, Nonmammalian / cytology
Embryo, Nonmammalian / metabolism
Gene Deletion
Gene Expression Regulation, Developmental
Genes, Helminth*
Green Fluorescent Proteins
Histones / metabolism
Luminescent Proteins / metabolism
Metaphase / genetics
Molecular Sequence Data
Phosphoprotein Phosphatases / genetics
Phosphoprotein Phosphatases / metabolism*
Phosphorylation
Protein Phosphatase 1
RNA, Double-Stranded / metabolism
RNA, Small Interfering / metabolism
Sequence Homology, Amino Acid
|
| IF |
3.383
|
| 引用数 |
6
|
|
WOS 分野
|
ONCOLOGY
CELL BIOLOGY
|
| リソース情報 |
| 線虫 |
tm301 |
