| RRC ID |
414
|
| 著者 |
Kimura Y, Corcoran EE, Eto K, Gengyo-Ando K, Muramatsu MA, Kobayashi R, Freedman JH, Mitani S, Hagiwara M, Means AR, Tokumitsu H.
|
| タイトル |
A CaMK cascade activates CRE-mediated transcription in neurons of Caenorhabditis elegans.
|
| ジャーナル |
EMBO Rep
|
| Abstract |
Calcium (Ca2+) signals regulate a diverse set of cellular responses, from proliferation to muscular contraction and neuro-endocrine secretion. The ubiquitous Ca2+ sensor, calmodulin (CaM), translates changes in local intracellular Ca2+ concentrations into changes in enzyme activities. Among its targets, the Ca2+/CaM-dependent protein kinases I and IV (CaMKs) are capable of transducing intraneuronal signals, and these kinases are implicated in neuronal gene regulation that mediates synaptic plasticity in mammals. Recently, the cyclic AMP response element binding protein (CREB) has been proposed as a target for a CaMK cascade involving not only CaMKI or CaMKIV, but also an upstream kinase kinase that is also CaM regulated (CaMKK). Here, we report that all components of this pathway are coexpressed in head neurons of Caenorhabditis elegans. Utilizing a transgenic approach to visualize CREB-dependent transcription in vivo, we show that this CaMK cascade regulates CRE-mediated transcription in a subset of head neurons in living nematodes.
|
| 巻・号 |
3(10)
|
| ページ |
962-6
|
| 公開日 |
2002-10-1
|
| DOI |
10.1093/embo-reports/kvf191
|
| PII |
kvf191
|
| PMID |
12231504
|
| PMC |
PMC1307624
|
| MeSH |
Amino Acid Sequence
Animals
Animals, Genetically Modified
Blotting, Western
Caenorhabditis elegans / genetics*
Caenorhabditis elegans / metabolism
Caenorhabditis elegans / physiology*
Calcium / metabolism
Calcium-Calmodulin-Dependent Protein Kinases / metabolism*
Cyclic AMP Response Element-Binding Protein / metabolism*
DNA, Complementary / metabolism
Electrophoresis, Polyacrylamide Gel
Exons
Gene Library
Humans
Mice
Molecular Sequence Data
Mutagenesis
Neurons / metabolism
Open Reading Frames
Phosphorylation
Reverse Transcriptase Polymerase Chain Reaction
Sequence Homology, Amino Acid
Signal Transduction
Transcription, Genetic
|
| IF |
7.497
|
| 引用数 |
78
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| 線虫 |
tm421 |
