論文 - 詳細
| RRC ID | 40023 |
|---|---|
| 著者 | Le TP, Vuong LT, Kim AR, Hsu YC, Choi KW. |
| タイトル | 14-3-3 proteins regulate Tctp-Rheb interaction for organ growth in Drosophila. |
| ジャーナル | Nat Commun |
| Abstract |
14-3-3 family proteins regulate multiple signalling pathways. Understanding biological functions of 14-3-3 proteins has been limited by the functional redundancy of conserved isotypes. Here we provide evidence that 14-3-3 proteins regulate two interacting components of Tor signalling in Drosophila, translationally controlled tumour protein (Tctp) and Rheb GTPase. Single knockdown of 14-3-3ɛ or 14-3-3ζ isoform does not show obvious defects in organ development but causes synergistic genetic interaction with Tctp and Rheb to impair tissue growth. 14-3-3 proteins physically interact with Tctp and Rheb. Knockdown of both 14-3-3 isoforms abolishes the binding between Tctp and Rheb, disrupting organ development. Depletion of 14-3-3s also reduces the level of phosphorylated S6 kinase, phosphorylated Thor/4E-BP and cyclin E (CycE). Growth defects from knockdown of 14-3-3 and Tctp are suppressed by CycE overexpression. This study suggests a novel mechanism of Tor regulation mediated by 14-3-3 interaction with Tctp and Rheb. |
| 巻・号 | 7 |
| ページ | 11501 |
| 公開日 | 2016-5-6 |
| DOI | 10.1038/ncomms11501 |
| PII | ncomms11501 |
| PMID | 27151460 |
| PMC | PMC4859069 |
| MeSH | 14-3-3 Proteins / genetics* Animals Biomarkers, Tumor / metabolism* Drosophila / embryology* Drosophila / genetics Drosophila Proteins / genetics* Drosophila Proteins / metabolism* Gene Expression Regulation, Developmental / genetics* Gene Knockdown Techniques Intracellular Signaling Peptides and Proteins Ras Homolog Enriched in Brain Protein / metabolism* Signal Transduction TOR Serine-Threonine Kinases / genetics* |
| IF | 12.121 |
| 引用数 | 13 |
| WOS 分野 | CELL BIOLOGY |
| リソース情報 | |
| ショウジョウバエ | 31196R-4 31196R-3 17870R-2 |