RRC ID 3744
著者 Smolikov S, Schild-Prüfert K, Colaiácovo MP.
タイトル CRA-1 uncovers a double-strand break-dependent pathway promoting the assembly of central region proteins on chromosome axes during C. elegans meiosis.
ジャーナル PLoS Genet
Abstract The synaptonemal complex (SC), a tripartite proteinaceous structure that forms between homologous chromosomes during meiosis, is crucial for faithful chromosome segregation. Here we identify CRA-1, a novel and conserved protein that is required for the assembly of the central region of the SC during C. elegans meiosis. In the absence of CRA-1, central region components fail to extensively localize onto chromosomes at early prophase and instead mostly surround the chromatin at this stage. Later in prophase, central region proteins polymerize along chromosome axes, but for the most part fail to connect the axes of paired homologous chromosomes. This defect results in an inability to stabilize homologous pairing interactions, altered double-strand break (DSB) repair progression, and a lack of chiasmata. Surprisingly, DSB formation and repair are required to promote the polymerization of the central region components along meiotic chromosome axes in cra-1 mutants. In the absence of both CRA-1 and any one of the C. elegans homologs of SPO11, MRE11, RAD51, or MSH5, the polymerization observed along chromosome axes is perturbed, resulting in the formation of aggregates of the SC central region proteins. While radiation-induced DSBs rescue this polymerization in cra-1; spo-11 mutants, they fail to do so in cra-1; mre-11, cra-1; rad-51, and cra-1; msh-5 mutants. Taken together, our studies place CRA-1 as a key component in promoting the assembly of a tripartite SC structure. Moreover, they reveal a scenario in which DSB formation and repair can drive the polymerization of SC components along chromosome axes in C. elegans.
巻・号 4(6)
ページ e1000088
公開日 2008-6-6
DOI 10.1371/journal.pgen.1000088
PMID 18535664
PMC PMC2408554
MeSH Amino Acid Sequence Animals Caenorhabditis elegans / cytology* Caenorhabditis elegans / genetics* Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / chemistry Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Chromosome Pairing Chromosomes / genetics* Crossing Over, Genetic DNA Breaks, Double-Stranded* / radiation effects Meiosis* Molecular Sequence Data Mutation Protein Structure, Tertiary Sequence Alignment Synaptonemal Complex / genetics* Synaptonemal Complex / metabolism
IF 5.175
引用数 35
WOS 分野 GENETICS & HEREDITY
リソース情報
線虫 tm2144