RRC ID |
36334
|
著者 |
Hirakawa I, Miyagawa S, Katsu Y, Kagami Y, Tatarazako N, Kobayashi T, Kusano T, Mizutani T, Ogino Y, Takeuchi T, Ohta Y, Iguchi T.
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タイトル |
Gene expression profiles in the testis associated with testis-ova in adult Japanese medaka (Oryziaslatipes) exposed to 17α-ethinylestradiol.
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ジャーナル |
Chemosphere
|
Abstract |
The occurrence of oocytes in the testis (testis-ova) of several fish species is often associated with exposure of estrogenic chemicals. However, induction mechanisms of the testis-ova remain to be elucidated. To develop marker genes for detecting testis-ova in the testis, adult male medaka were exposed to nominal concentration of 100 ng L(-1) of 17α-ethinylestradiol (EE2) for 3-5 weeks, and 800 ng estradiol benzoate (EB) for 3 weeks (experiment I), and a measured concentration of 20 ng L(-1) EE2 for 1-6 weeks (experiment II). Histological analysis was performed for the testis, and microarray analyses were performed for the testis, liver and brain. Microarray analysis in the estrogen-exposed medaka liver showed vitellogenin and choriogenin as estrogen responsive genes. Testis-ova were induced in the testis after 4 weeks of exposure to 100 ng L(-1) EE2, 3 weeks of exposure to 800 ng EB, and 6 weeks of exposure to 20 ng L(-1) EE2. Microarray analysis of estrogen-exposed testes revealed up-regulation of genes related to zona pellucida (ZP) and the oocytes marker gene, 42Sp50. Using quantitative RT-PCR we confirmed that Zpc5 gene can be used as a marker for the detection of testis-ova in male medaka.
|
巻・号 |
87(7)
|
ページ |
668-74
|
公開日 |
2012-5-1
|
DOI |
10.1016/j.chemosphere.2011.12.047
|
PII |
S0045-6535(11)01416-0
|
PMID |
22230730
|
MeSH |
Animals
Endocrine Disruptors / metabolism
Endocrine Disruptors / toxicity
Ethinyl Estradiol / metabolism
Ethinyl Estradiol / toxicity*
Fish Proteins / genetics
Gene Expression Profiling
Liver / metabolism
Male
Oocytes
Ovum
Testis / drug effects
Testis / growth & development
Testis / metabolism*
Testis / pathology
Vitellogenins / metabolism
Water Pollutants, Chemical / metabolism
Water Pollutants, Chemical / toxicity*
|
IF |
5.778
|
引用数 |
29
|
WOS 分野
|
ENVIRONMENTAL SCIENCES
|
リソース情報 |
メダカ |
HdrR-II1(IB178) |