RRC ID 36334
著者 Hirakawa I, Miyagawa S, Katsu Y, Kagami Y, Tatarazako N, Kobayashi T, Kusano T, Mizutani T, Ogino Y, Takeuchi T, Ohta Y, Iguchi T.
タイトル Gene expression profiles in the testis associated with testis-ova in adult Japanese medaka (Oryziaslatipes) exposed to 17α-ethinylestradiol.
ジャーナル Chemosphere
Abstract The occurrence of oocytes in the testis (testis-ova) of several fish species is often associated with exposure of estrogenic chemicals. However, induction mechanisms of the testis-ova remain to be elucidated. To develop marker genes for detecting testis-ova in the testis, adult male medaka were exposed to nominal concentration of 100 ng L(-1) of 17α-ethinylestradiol (EE2) for 3-5 weeks, and 800 ng estradiol benzoate (EB) for 3 weeks (experiment I), and a measured concentration of 20 ng L(-1) EE2 for 1-6 weeks (experiment II). Histological analysis was performed for the testis, and microarray analyses were performed for the testis, liver and brain. Microarray analysis in the estrogen-exposed medaka liver showed vitellogenin and choriogenin as estrogen responsive genes. Testis-ova were induced in the testis after 4 weeks of exposure to 100 ng L(-1) EE2, 3 weeks of exposure to 800 ng EB, and 6 weeks of exposure to 20 ng L(-1) EE2. Microarray analysis of estrogen-exposed testes revealed up-regulation of genes related to zona pellucida (ZP) and the oocytes marker gene, 42Sp50. Using quantitative RT-PCR we confirmed that Zpc5 gene can be used as a marker for the detection of testis-ova in male medaka.
巻・号 87(7)
ページ 668-74
公開日 2012-5-1
DOI 10.1016/j.chemosphere.2011.12.047
PII S0045-6535(11)01416-0
PMID 22230730
MeSH Animals Endocrine Disruptors / metabolism Endocrine Disruptors / toxicity Ethinyl Estradiol / metabolism Ethinyl Estradiol / toxicity* Fish Proteins / genetics Gene Expression Profiling Liver / metabolism Male Oocytes Ovum Testis / drug effects Testis / growth & development Testis / metabolism* Testis / pathology Vitellogenins / metabolism Water Pollutants, Chemical / metabolism Water Pollutants, Chemical / toxicity*
IF 5.778
引用数 29
WOS 分野 ENVIRONMENTAL SCIENCES
リソース情報
メダカ HdrR-II1(IB178)