RRC ID 3629
著者 Sasagawa Y, Yamanaka K, Ogura T.
タイトル ER E3 ubiquitin ligase HRD-1 and its specific partner chaperone BiP play important roles in ERAD and developmental growth in Caenorhabditis elegans.
ジャーナル Genes Cells
Abstract p97 (also called VCP or Cdc48p) and E3 ubiquitin ligases are the key players in retrotranslocation and ubiquitination of substrates in the endoplasmic reticulum-associated degradation (ERAD) pathways. Although their biochemical properties have been well studied, their cellular functions in development have not been revealed. Here, we investigate cellular functions of p97 and E3 ubiquitin ligases in Caenorhabditis elegans as a model organism. We found that C. elegans possesses three E3 ubiquitin ligases (named as HRD-1, HRDL-1 and MARC-6) like mammals, and that their simultaneous depletion caused extremely delayed growth. By monitoring the expression of an ER chaperone gene, it was revealed that p97 and HRD-1 play essential roles in unfolded protein response (UPR) and ERAD pathways. We further found that HRD-1 functions in concert with BiP, and that two BiP paralogues are functionally diversified. HRD-1 and BiP(HSP-3) play important roles in the developmental growth and function of intestinal cells, while HRD-1 and BiP(HSP-4) in the gonad formation. We propose that E3 ubiquitin ligases function in concert with a specific partner chaperone.
巻・号 12(9)
ページ 1063-73
公開日 2007-9-1
DOI 10.1111/j.1365-2443.2007.01108.x
PII GTC1108
PMID 17825049
MeSH Adaptor Proteins, Signal Transducing / genetics Adaptor Proteins, Signal Transducing / physiology* Adenosine Triphosphatases / metabolism Animals Caenorhabditis elegans / growth & development* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism Caenorhabditis elegans Proteins / physiology* Cell Cycle Proteins / metabolism Endoplasmic Reticulum / enzymology Endoplasmic Reticulum / metabolism* Models, Genetic Molecular Chaperones / physiology* Phenotype Phylogeny Ubiquitin-Protein Ligases / genetics Ubiquitin-Protein Ligases / metabolism Ubiquitin-Protein Ligases / physiology* Valosin Containing Protein
IF 1.655
引用数 33
WOS 分野 GENETICS & HEREDITY CELL BIOLOGY
リソース情報
線虫 tm1743 tm544 tm659 tm1173 tm1380 tm1180