RRC ID |
3607
|
著者 |
Mukhopadhyay S, Lu Y, Qin H, Lanjuin A, Shaham S, Sengupta P.
|
タイトル |
Distinct IFT mechanisms contribute to the generation of ciliary structural diversity in C. elegans.
|
ジャーナル |
EMBO J
|
Abstract |
Individual cell types can elaborate morphologically diverse cilia. Cilia are assembled via intraflagellar transport (IFT) of ciliary precursors; however, the mechanisms that generate ciliary diversity are unknown. Here, we examine IFT in the structurally distinct cilia of the ASH/ASI and the AWB chemosensory neurons in Caenorhabditis elegans, enabling us to compare IFT in specific cilia types. We show that unlike in the ASH/ASI cilia, the OSM-3 kinesin moves independently of the kinesin-II motor in the AWB cilia. Although OSM-3 is essential to extend the distal segments of the ASH/ASI cilia, it is not required to build the AWB distal segments. Mutations in the fkh-2 forkhead domain gene result in AWB-specific defects in ciliary morphology, and FKH-2 regulates kinesin-II subunit gene expression specifically in AWB. Our results suggest that cell-specific regulation of IFT contributes to the generation of ciliary diversity, and provide insights into the networks coupling the acquisition of ciliary specializations with other aspects of cell fate.
|
巻・号 |
26(12)
|
ページ |
2966-80
|
公開日 |
2007-6-20
|
DOI |
10.1038/sj.emboj.7601717
|
PII |
7601717
|
PMID |
17510633
|
PMC |
PMC1894762
|
MeSH |
Animals
Animals, Genetically Modified
Biological Transport
Caenorhabditis elegans / cytology*
Caenorhabditis elegans Proteins / physiology
Cilia / ultrastructure*
Kinesins / physiology
Microscopy, Electron
Mutation
Olfactory Receptor Neurons / ultrastructure
|
IF |
9.889
|
引用数 |
65
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
リソース情報 |
線虫 |
tm324 |