RRC ID 3602
著者 Maruyama R, Velarde NV, Klancer R, Gordon S, Kadandale P, Parry JM, Hang JS, Rubin J, Stewart-Michaelis A, Schweinsberg P, Grant BD, Piano F, Sugimoto A, Singson A.
タイトル EGG-3 regulates cell-surface and cortex rearrangements during egg activation in Caenorhabditis elegans.
ジャーナル Curr Biol
Abstract Fertilization triggers egg activation and converts the egg into a developing embryo. The events of this egg-to-embryo transition typically include the resumption of meiosis, the reorganization of the cortical actin cytoskeleton, and the remodeling of the oocyte surface. The factors that regulate sperm-dependent egg-activation events are not well understood. Caenorhabditis elegans EGG-3, a member of the protein tyrosine phosphatase-like (PTPL) family, is essential for regulating cell-surface and cortex rearrangements during egg activation in response to sperm entry. Although fertilization occurred normally in egg-3 mutants, the polarized dispersal of F-actin is altered, a chitin eggshell is not formed, and no polar bodies are produced. EGG-3 is associated with the oocyte plasma membrane in a pattern that is similar to CHS-1 and MBK-2. CHS-1 is required for eggshell deposition, whereas MBK-2 is required for the degradation of maternal proteins during the egg-to-embryo transition. The localization of CHS-1 and EGG-3 are interdependent and both genes were required for the proper localization of MBK-2 in oocytes. Therefore, EGG-3 plays a central role in egg activation by influencing polarized F-actin dynamics and the localization or activity of molecules that are directly involved in executing the egg-to-embryo transition.
巻・号 17(18)
ページ 1555-60
公開日 2007-9-18
DOI 10.1016/j.cub.2007.08.011
PII S0960-9822(07)01787-3
PMID 17869112
MeSH Actins / analysis Actins / metabolism Amino Acid Motifs Animals Caenorhabditis elegans / embryology* Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / analysis Caenorhabditis elegans Proteins / chemistry Caenorhabditis elegans Proteins / metabolism Caenorhabditis elegans Proteins / physiology* Cell Membrane / metabolism Embryo, Nonmammalian / cytology Embryo, Nonmammalian / metabolism Fertilization Green Fluorescent Proteins / analysis Molecular Sequence Data Ovum / cytology Ovum / growth & development* Ovum / metabolism Protein Tyrosine Phosphatases / chemistry Protein Tyrosine Phosphatases / metabolism Protein Tyrosine Phosphatases / physiology* Protein-Tyrosine Kinases / analysis Protein-Tyrosine Kinases / metabolism
IF 9.601
引用数 51
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
線虫 tm1191