| RRC ID |
36017
|
| 著者 |
Hirata T, Cabrero P, Berkholz DS, Bondeson DP, Ritman EL, Thompson JR, Dow JA, Romero MF.
|
| タイトル |
In vivo Drosophilia genetic model for calcium oxalate nephrolithiasis.
|
| ジャーナル |
Am J Physiol Renal Physiol
|
| Abstract |
Nephrolithiasis is a major public health problem with a complex and varied etiology. Most stones are composed of calcium oxalate (CaOx), with dietary excess a risk factor. Because of complexity of mammalian system, the details of stone formation remain to be understood. Here we have developed a nephrolithiasis model using the genetic model Drosophila melanogaster, which has a simple, transparent kidney tubule. Drosophilia reliably develops CaOx stones upon dietary oxalate supplementation, and the nucleation and growth of microliths can be viewed in real time. The Slc26 anion transporter dPrestin (Slc26a5/6) is strongly expressed in Drosophilia kidney, and biophysical analysis shows that it is a potent oxalate transporter. When dPrestin is knocked down by RNAi in fly kidney, formation of microliths is reduced, identifying dPrestin as a key player in oxalate excretion. CaOx stone formation is an ancient conserved process across >400 My of divergent evolution (fly and human), and from this study we can conclude that the fly is a good genetic model of nephrolithiasis.
|
| 巻・号 |
303(11)
|
| ページ |
F1555-62
|
| 公開日 |
2012-12-1
|
| DOI |
10.1152/ajprenal.00074.2012
|
| PII |
ajprenal.00074.2012
|
| PMID |
22993075
|
| PMC |
PMC3532482
|
| MeSH |
Animals
Animals, Genetically Modified
Anion Transport Proteins / genetics
Anion Transport Proteins / metabolism
Calcium Oxalate / metabolism
Drosophila / genetics*
Gene Knockdown Techniques
Kidney Tubules / metabolism
Models, Animal*
Models, Genetic*
Nephrolithiasis / genetics*
Nephrolithiasis / metabolism
|
| IF |
3.191
|
| 引用数 |
27
|
|
WOS 分野
|
UROLOGY & NEPHROLOGY
PHYSIOLOGY
|
| リソース情報 |
| ショウジョウバエ |
5485R-3 |
