RRC ID 35976
著者 Agrawal T, Hasan G.
タイトル Maturation of a central brain flight circuit in Drosophila requires Fz2/Ca²⁺ signaling.
ジャーナル Elife
Abstract The final identity of a differentiated neuron is determined by multiple signaling events, including activity dependent calcium transients. Non-canonical Frizzled2 (Fz2) signaling generates calcium transients that determine neuronal polarity, neuronal migration, and synapse assembly in the developing vertebrate brain. Here, we demonstrate a requirement for Fz2/Ca(2+) signaling in determining the final differentiated state of a set of central brain dopaminergic neurons in Drosophila, referred to as the protocerebral anterior medial (PAM) cluster. Knockdown or inhibition of Fz2/Ca(2+) signaling during maturation of the flight circuit in pupae reduces Tyrosine Hydroxylase (TH) expression in the PAM neurons and affects maintenance of flight. Thus, we demonstrate that Fz2/Ca(2+) transients during development serve as a pre-requisite for normal adult behavior. Our results support a neural mechanism where PAM neuron send projections to the α' and β' lobes of a higher brain centre, the mushroom body, and function in dopaminergic re-inforcement of flight.
巻・号 4
公開日 2015-5-8
DOI 10.7554/eLife.07046
PMID 25955970
PMC PMC4451221
MeSH Animals Brain / growth & development Brain / metabolism Calcium / metabolism* Calcium Signaling* Cell Movement Cell Polarity Dopaminergic Neurons / cytology Dopaminergic Neurons / metabolism Drosophila Proteins / genetics* Drosophila Proteins / metabolism Drosophila melanogaster / genetics Drosophila melanogaster / growth & development Drosophila melanogaster / metabolism Flight, Animal / physiology Frizzled Receptors / genetics* Frizzled Receptors / metabolism Gene Expression Regulation, Developmental Genetic Complementation Test Inositol 1,4,5-Trisphosphate Receptors / genetics Inositol 1,4,5-Trisphosphate Receptors / metabolism Membrane Proteins / genetics Membrane Proteins / metabolism Mushroom Bodies / growth & development Mushroom Bodies / metabolism Pupa / genetics Pupa / growth & development Pupa / metabolism Stromal Interaction Molecule 1 Synapses / physiology Tyrosine 3-Monooxygenase / genetics Tyrosine 3-Monooxygenase / metabolism
IF 7.08
引用数 15
リソース情報
ショウジョウバエ 1063R-2