RRC ID 35954
著者 Clavier A, Rincheval-Arnold A, Baillet A, Mignotte B, Guénal I.
タイトル Two different specific JNK activators are required to trigger apoptosis or compensatory proliferation in response to Rbf1 in Drosophila.
ジャーナル Cell Cycle
Abstract The Jun Kinase (JNK) signaling pathway responds to diverse stimuli by appropriate and specific cellular responses such as apoptosis, differentiation or proliferation. The mechanisms that mediate this specificity remain largely unknown. The core of this signaling pathway, composed of a JNK protein and a JNK kinase (JNKK), can be activated by various putative JNKK kinases (JNKKK) which are themselves downstream of different adaptor proteins. A proposed hypothesis is that the JNK pathway specific response lies in the combination of a JNKKK and an adaptor protein upstream of the JNKK. We previously showed that the Drosophila homolog of pRb (Rbf1) and a mutant form of Rbf1 (Rbf1(D253A)) have JNK-dependent pro-apoptotic properties. Rbf1(D253A) is also able to induce a JNK-dependent abnormal proliferation. Here, we show that Rbf1-induced apoptosis triggers proliferation which depends on the JNK pathway activation. Taking advantage of these phenotypes, we investigated the JNK signaling involved in either Rbf1-induced apoptosis or in proliferation in response to Rbf1-induced apoptosis. We demonstrated that 2 different JNK pathways involving different adaptor proteins and kinases are involved in Rbf1-apoptosis (i.e. Rac1-dTak1-dMekk1-JNK pathway) and in proliferation in response to Rbf1-induced apoptosis (i.e., dTRAF1-Slipper-JNK pathway). Using a transient induction of rbf1, we show that Rbf1-induced apoptosis activates a compensatory proliferation mechanism which also depends on Slipper and dTRAF1. Thus, these 2 proteins seem to be key players of compensatory proliferation in Drosophila.
巻・号 15(2)
ページ 283-94
公開日 2016-1-29
DOI 10.1080/15384101.2015.1100776
PMID 26825229
PMC PMC4825841
MeSH Animals Apoptosis / genetics* Cell Proliferation / genetics* Drosophila Proteins / genetics* Drosophila Proteins / metabolism Drosophila melanogaster / genetics* Drosophila melanogaster / metabolism Gene Expression Regulation MAP Kinase Kinase Kinases / genetics* MAP Kinase Kinase Kinases / metabolism MAP Kinase Signaling System Retinoblastoma Protein TNF Receptor-Associated Factor 1 / genetics* TNF Receptor-Associated Factor 1 / metabolism Transcription Factors / genetics* Transcription Factors / metabolism rac GTP-Binding Proteins / genetics rac GTP-Binding Proteins / metabolism
IF 3.699
引用数 5
WOS 分野 CELL BIOLOGY
リソース情報
ショウジョウバエ 4353R-2