RRC ID 3589
著者 Hughes S, Stürzenbaum SR.
タイトル Single and double metallothionein knockout in the nematode C. elegans reveals cadmium dependent and independent toxic effects on life history traits.
ジャーナル Environ Pollut
Abstract The genome of the nematode Caenorhabditis elegans contains two metallothionein genes, both involved in metal homeostasis and/or detoxification. Single metallothionein knockout mutants have been created and now, for the first time, a double mutant has been isolated. Life history studies in the presence or absence of cadmium showed that all metallothionein mutants are viable. Although cadmium did not influence longevity, a dose dependent reduction in total brood size and volumetric growth was observed in wild type animals, which was magnified in single knockouts and further exacerbated in the double knockout. However, the metallothionein deletion caused two effects that are independent of cadmium exposure, namely all knockout strains displayed a reduced total brood size and the deletion of both metallothionein loci caused a significant reduction in volumetric growth. In summary, metallothionein is undoubtedly an important player in cadmium detoxification, but evidently also an important factor in cadmium independent pathways.
巻・号 145(2)
ページ 395-400
公開日 2007-1-1
DOI 10.1016/j.envpol.2006.06.003
PII S0269-7491(06)00372-1
PMID 17141712
MeSH Animals Cadmium / toxicity* Caenorhabditis elegans / genetics* Caenorhabditis elegans / growth & development Environmental Pollutants / toxicity* Gene Deletion Life Cycle Stages / genetics Longevity / genetics Metallothionein / genetics* Mutation Reproduction / genetics
IF 6.793
引用数 40
WOS 分野 ENVIRONMENTAL SCIENCES
リソース情報
線虫 tm1770 tm1700