RRC ID 3574
著者 Gomez TA, Banfield KL, Trogler DM, Clarke SG.
タイトル The L-isoaspartyl-O-methyltransferase in Caenorhabditis elegans larval longevity and autophagy.
ジャーナル Dev Biol
Abstract The protein L-isoaspartyl-O-methyltransferase, coded by the pcm-1 gene in Caenorhabditis elegans, participates in the repair of age-damaged proteins. We tested the ability of pcm-1-deficient nematodes to survive starvation stress as developmentally-arrested L1 larvae. We found that pcm-1 mutant L1 larvae do not survive as well as wild-type L1 larvae when incubated in M9 medium without nutrients. We then tested whether the starved L1 larvae could continue development when allowed access to food in a recovery assay. A loss of recovery ability with age was observed for all larvae, with little or no difference between the pcm-1 mutant and wild-type N2 larvae. Interestingly, when L1 larvae were starved in cholesterol-containing S medium or M9 medium supplemented with cholesterol, the survival rates of both mutant and wild-type animals nearly doubles, with pcm-1 larvae again faring more poorly than N2 larvae. Furthermore, L1 larvae cultured in these cholesterol-containing media show an increase in Sudan Black staining over animals cultured in M9 medium. The longevity defects of pcm-1 mutants previously seen in dauer larvae and here in L1 larvae suggest a defect in the ability of pcm-1 mutants to recycle and reuse old cellular components in pathways such as autophagy. Using an autophagosomal marker, we found evidence suggesting that the pcm-1 mutation may inhibit autophagy during dauer formation, suggesting that the absence of protein repair may also interfere with protein degradation pathways.
巻・号 303(2)
ページ 493-500
公開日 2007-3-15
DOI 10.1016/j.ydbio.2006.11.023
PII S0012-1606(06)01378-9
PMID 17187774
PMC PMC1868680
MeSH Animals Autophagy / genetics Autophagy / physiology* Caenorhabditis elegans / enzymology Caenorhabditis elegans / genetics Caenorhabditis elegans / growth & development Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / physiology* Cell Cycle Proteins / genetics Cell Cycle Proteins / physiology* Cholesterol / metabolism Genes, Helminth Larva / enzymology Longevity / genetics Longevity / physiology* Methyltransferases / genetics Methyltransferases / physiology* Mutation Starvation
IF 2.896
引用数 19
WOS 分野 DEVELOPMENTAL BIOLOGY
リソース情報
線虫 tm363