RRC ID 3549
著者 Stavru F, Hülsmann BB, Spang A, Hartmann E, Cordes VC, Görlich D.
タイトル NDC1: a crucial membrane-integral nucleoporin of metazoan nuclear pore complexes.
ジャーナル J Cell Biol
Abstract POM121 and gp210 were, until this point, the only known membrane-integral nucleoporins (Nups) of vertebrates and, thus, the only candidate anchors for nuclear pore complexes (NPCs) within the nuclear membrane. In an accompanying study (Stavru et al.), we provided evidence that NPCs can exist independently of POM121 and gp210, and we predicted that vertebrate NPCs contain additional membrane-integral constituents. We identify such an additional membrane protein in the NPCs of mammals, frogs, insects, and nematodes as the orthologue to yeast Ndc1p/Cut11p. Human NDC1 (hNDC1) likely possesses six transmembrane segments, and it is located at the nuclear pore wall. Depletion of hNDC1 from human HeLa cells interferes with the assembly of phenylalanine-glycine repeat Nups into NPCs. The loss of NDC1 function in Caenorhabditis elegans also causes severe NPC defects and very high larval and embryonic mortality. However, it is not ultimately lethal. Instead, homozygous NDC1-deficient worms can be propagated. This indicates that none of the membrane-integral Nups is universally essential for NPC assembly, and suggests that NPC biogenesis is an extremely fault-tolerant process.
巻・号 173(4)
ページ 509-19
公開日 2006-5-22
DOI 10.1083/jcb.200601001
PII jcb.200601001
PMID 16702233
PMC PMC2063861
MeSH Amino Acid Sequence / genetics Animals Caenorhabditis elegans Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Conserved Sequence / genetics Evolution, Molecular Gene Expression Regulation, Developmental / genetics Genes, Lethal / genetics HeLa Cells Humans Larva / genetics Larva / growth & development Mice Molecular Sequence Data Nuclear Pore / genetics Nuclear Pore / metabolism* Nuclear Pore / ultrastructure Nuclear Pore Complex Proteins / chemistry Nuclear Pore Complex Proteins / genetics Nuclear Pore Complex Proteins / metabolism* Protein Structure, Tertiary / genetics Sequence Homology, Amino Acid
IF 8.811
引用数 105
WOS 分野 CELL BIOLOGY
リソース情報
線虫 tm1845