RRC ID 3537
著者 Prasad BC, Clark SG.
タイトル Wnt signaling establishes anteroposterior neuronal polarity and requires retromer in C. elegans.
ジャーナル Development
Abstract Secreted Wnt proteins influence neural connectivity by regulating axon guidance, dendritic morphogenesis and synapse formation. We report a new role for Wnt and Frizzled proteins in establishing the anteroposterior polarity of the mechanosensory neurons ALM and PLM in C. elegans. Disruption of Wnt signaling leads to a complete inversion of ALM and PLM polarity: the anterior process adopts the length, branching pattern and synaptic properties of the wild-type posterior process, and vice versa. Different but overlapping sets of Wnt proteins regulate neuronal polarity in different body regions. Wnts act directly on PLM via the Frizzled LIN-17. In addition, we show that they are needed for axon branching and anteriorly directed axon growth. We also find that the retromer, a conserved protein complex that mediates transcytosis and endosome-to-Golgi protein trafficking, plays a key role in Wnt signaling. Deletion mutations of retromer subunits cause ALM and PLM polarity, and other Wnt-related defects. We show that retromer protein VPS-35 is required in Wnt-expressing cells and propose that retromer activity is needed to generate a fully active Wnt signal.
巻・号 133(9)
ページ 1757-66
公開日 2006-5-1
DOI 10.1242/dev.02357
PII dev.02357
PMID 16571624
MeSH Animals Caenorhabditis elegans / cytology Caenorhabditis elegans / genetics Caenorhabditis elegans / growth & development Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / physiology* Cell Polarity* Green Fluorescent Proteins / metabolism Models, Biological Mutation Neurons, Afferent / cytology Neurons, Afferent / physiology RNA Interference Receptors, G-Protein-Coupled / genetics Receptors, G-Protein-Coupled / physiology* Signal Transduction* Vesicular Transport Proteins / genetics Wnt Proteins / genetics Wnt Proteins / physiology*
IF 5.611
引用数 160
WOS 分野 DEVELOPMENTAL BIOLOGY
リソース情報
線虫 tm1320 tm1523