RRC ID 35156
著者 Yamanaka N, Marqués G, O'Connor MB.
タイトル Vesicle-Mediated Steroid Hormone Secretion in Drosophila melanogaster.
ジャーナル Cell
Abstract Steroid hormones are a large family of cholesterol derivatives regulating development and physiology in both the animal and plant kingdoms, but little is known concerning mechanisms of their secretion from steroidogenic tissues. Here, we present evidence that in Drosophila, endocrine release of the steroid hormone ecdysone is mediated through a regulated vesicular trafficking mechanism. Inhibition of calcium signaling in the steroidogenic prothoracic gland results in the accumulation of unreleased ecdysone, and the knockdown of calcium-mediated vesicle exocytosis components in the gland caused developmental defects due to deficiency of ecdysone. Accumulation of synaptotagmin-labeled vesicles in the gland is observed when calcium signaling is disrupted, and these vesicles contain an ABC transporter that functions as an ecdysone pump to fill vesicles. We propose that trafficking of steroid hormones out of endocrine cells is not always through a simple diffusion mechanism as presently thought, but instead can involve a regulated vesicle-mediated release process.
巻・号 163(4)
ページ 907-19
公開日 2015-11-5
DOI 10.1016/j.cell.2015.10.022
PII S0092-8674(15)01336-7
PMID 26544939
PMC PMC4636736
MeSH ATP-Binding Cassette Transporters / metabolism Adenosine Triphosphate / metabolism Animals Diffusion Drosophila Proteins / metabolism Drosophila melanogaster / cytology Drosophila melanogaster / growth & development Drosophila melanogaster / metabolism* Ecdysone / metabolism* Endocrine Glands / metabolism Exocytosis Gene Knockdown Techniques Inositol 1,4,5-Trisphosphate Receptors / genetics Inositol 1,4,5-Trisphosphate Receptors / metabolism Larva / cytology Larva / metabolism Secretory Vesicles / metabolism* Synaptotagmins / metabolism
IF 38.637
引用数 42
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
ショウジョウバエ 5562R-1