RRC ID 34871
著者 Zhou B, Lindsay SA, Wasserman SA.
タイトル Alternative NF-κB Isoforms in the Drosophila Neuromuscular Junction and Brain.
ジャーナル PLoS One
Abstract The Drosophila NF-κB protein Dorsal is expressed at the larval neuromuscular junction, where its expression appears unrelated to known Dorsal functions in embryonic patterning and innate immunity. Using confocal microscopy with domain-specific antisera, we demonstrate that larval muscle expresses only the B isoform of Dorsal, which arises by intron retention. We find that Dorsal B interacts with and stabilizes Cactus at the neuromuscular junction, but exhibits Cactus independent localization and an absence of detectable nuclear translocation. We further find that the Dorsal-related immune factor Dif encodes a B isoform, reflecting a conservation of B domains across a range of insect NF-κB proteins. Carrying out mutagenesis of the Dif locus via a site-specific recombineering approach, we demonstrate that Dif B is the major, if not sole, Dif isoform in the mushroom bodies of the larval brain. The Dorsal and Dif B isoforms thus share a specific association with nervous system tissues as well as an alternative protein structure.
巻・号 10(7)
ページ e0132793
公開日 2015-7-13
DOI 10.1371/journal.pone.0132793
PII PONE-D-15-21766
PMID 26167685
PMC PMC4500392
MeSH Animals Brain / metabolism* Drosophila Microscopy, Confocal NF-kappa B / metabolism* Neuromuscular Junction / metabolism*
IF 2.74
引用数 5
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ショウジョウバエ 5848R-3