| RRC ID |
34747
|
| 著者 |
Hsu JM, Chen CH, Chen YC, McDonald KL, Gurling M, Lee A, Garriga G, Pan CL.
|
| タイトル |
Genetic analysis of a novel tubulin mutation that redirects synaptic vesicle targeting and causes neurite degeneration in C. elegans.
|
| ジャーナル |
PLoS Genet
|
| Abstract |
Neuronal cargos are differentially targeted to either axons or dendrites, and this polarized cargo targeting critically depends on the interaction between microtubules and molecular motors. From a forward mutagenesis screen, we identified a gain-of-function mutation in the C. elegans α-tubulin gene mec-12 that triggered synaptic vesicle mistargeting, neurite swelling and neurodegeneration in the touch receptor neurons. This missense mutation replaced an absolutely conserved glycine in the H12 helix with glutamic acid, resulting in increased negative charges at the C-terminus of α-tubulin. Synaptic vesicle mistargeting in the mutant neurons was suppressed by reducing dynein function, suggesting that aberrantly high dynein activity mistargeted synaptic vesicles. We demonstrated that dynein showed preference towards binding mutant microtubules over wild-type in microtubule sedimentation assay. By contrast, neurite swelling and neurodegeneration were independent of dynein and could be ameliorated by genetic paralysis of the animal. This suggests that mutant microtubules render the neurons susceptible to recurrent mechanical stress induced by muscle activity, which is consistent with the observation that microtubule network was disorganized under electron microscopy. Our work provides insights into how microtubule-dynein interaction instructs synaptic vesicle targeting and the importance of microtubule in the maintenance of neuronal structures against constant mechanical stress.
|
| 巻・号 |
10(11)
|
| ページ |
e1004715
|
| 公開日 |
2014-11-1
|
| DOI |
10.1371/journal.pgen.1004715
|
| PII |
PGENETICS-D-14-00758
|
| PMID |
25392990
|
| PMC |
PMC4230729
|
| MeSH |
Animals
Caenorhabditis elegans
Caenorhabditis elegans Proteins / genetics*
Caenorhabditis elegans Proteins / metabolism
Dendrites / genetics
Dendrites / metabolism
Dendrites / pathology
Dyneins / metabolism
Exocytosis
Humans
Microtubules / metabolism
Mutation, Missense
Nerve Degeneration / genetics*
Nerve Degeneration / pathology
Neurites / metabolism
Neurites / pathology
Synaptic Transmission / genetics*
Synaptic Vesicles / genetics*
Synaptic Vesicles / metabolism
Tubulin / genetics*
Tubulin / metabolism
|
| IF |
5.175
|
| 引用数 |
8
|
|
WOS 分野
|
GENETICS & HEREDITY
|
| リソース情報 |
| 線虫 |
mec-12(tm5083) |
