RRC ID 3441
著者 Labbé JC, Pacquelet A, Marty T, Gotta M.
タイトル A genomewide screen for suppressors of par-2 uncovers potential regulators of PAR protein-dependent cell polarity in Caenorhabditis elegans.
ジャーナル Genetics
Abstract The PAR proteins play an essential role in establishing and maintaining cell polarity. While their function is conserved across species, little is known about their regulators and effectors. Here we report the identification of 13 potential components of the C. elegans PAR polarity pathway, identified in an RNAi-based, systematic screen to find suppressors of par-2(it5ts) lethality. Most of these genes are conserved in other species. Phenotypic analysis of double-mutant animals revealed that some of the suppressors can suppress lethality associated with the strong loss-of-function allele par-2(lw32), indicating that they might impinge on the PAR pathway independently of the PAR-2 protein. One of these is the gene nos-3, which encodes a homolog of Drosophila Nanos. We find that nos-3 suppresses most of the phenotypes associated with loss of par-2 function, including early cell division defects and maternal-effect sterility. Strikingly, while PAR-1 activity was essential in nos-3; par-2 double mutants, its asymmetric localization at the posterior cortex was not restored, suggesting that the function of PAR-1 is independent of its cortical localization. Taken together, our results identify conserved components that regulate PAR protein function and also suggest a role for NOS-3 in PAR protein-dependent cell polarity.
巻・号 174(1)
ページ 285-95
公開日 2006-9-1
DOI 10.1534/genetics.106.060517
PII genetics.106.060517
PMID 16816419
PMC PMC1569778
MeSH Animals Animals, Genetically Modified Body Patterning / genetics Caenorhabditis elegans / genetics* Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism Caenorhabditis elegans Proteins / physiology Cell Polarity / genetics* Chromosome Mapping / methods* Disorders of Sex Development Embryo, Nonmammalian Female Gene Expression Regulation, Developmental Genes, Lethal Genes, Suppressor* Male Protein Serine-Threonine Kinases / metabolism RNA Interference / physiology RNA, Small Interfering / physiology RNA-Binding Proteins / physiology Suppression, Genetic Tissue Distribution
IF 4.015
引用数 49
WOS 分野 GENETICS & HEREDITY
リソース情報
線虫 tm1180 tm1349 tm463 tm501 tm924