RRC ID 3418
著者 Clejan I, Boerckel J, Ahmed S.
タイトル Developmental modulation of nonhomologous end joining in Caenorhabditis elegans.
ジャーナル Genetics
Abstract Homologous recombination and nonhomologous end joining (NHEJ) are important DNA double-strand break repair pathways in many organisms. C. elegans strains harboring mutations in the cku-70, cku-80, or lig-4 NHEJ genes displayed multiple developmental abnormalities in response to radiation-induced DNA damage in noncycling somatic cells. These phenotypes did not result from S-phase, DNA damage, or mitotic checkpoints, apoptosis, or stress response pathways that regulate dauer formation. However, an additional defect in him-10, a kinetochore component, synergized with NHEJ mutations for the radiation-induced developmental phenotypes, suggesting that they may be triggered by mis-segregation of chromosome fragments. Although NHEJ was an important DNA repair pathway for noncycling somatic cells in C. elegans, homologous recombination was used to repair radiation-induced DNA damage in cycling somatic cells and in germ cells at all times. Noncycling germ cells that depended on homologous recombination underwent cell cycle arrest in G2, whereas noncycling somatic cells that depended on NHEJ arrested in G1, suggesting that cell cycle phase may modulate DNA repair during development. We conclude that error-prone NHEJ plays little or no role in DNA repair in C. elegans germ cells, possibly ensuring homology-based double-strand break repair and transmission of a stable genome from one generation to the next.
巻・号 173(3)
ページ 1301-17
公開日 2006-7-1
DOI 10.1534/genetics.106.058628
PII genetics.106.058628
PMID 16702421
PMC PMC1526663
MeSH Animals Caenorhabditis elegans / genetics* Caenorhabditis elegans / growth & development* DNA Repair* Gene Expression Regulation, Developmental* / physiology Germ Cells Kinetochores Larva Models, Biological Models, Genetic Mutation Phenotype Recombination, Genetic*
IF 4.015
引用数 67
WOS 分野 GENETICS & HEREDITY
リソース情報
線虫 tm1203 tm750 tm1524