論文 - 詳細
RRC ID | 3416 |
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著者 | Chesney MA, Kidd AR 3rd, Kimble J. |
タイトル | gon-14 functions with class B and class C synthetic multivulva genes to control larval growth in Caenorhabditis elegans. |
ジャーナル | Genetics |
Abstract |
Previous work showed that C. elegans gon-14 is required for gonadogenesis. Here we report that gon-14 encodes a protein with similarity to LIN-15B, a class B synMuv protein. An extensive region of GON-14 contains blocks of sequence similarity to transposases of the hAT superfamily, but key residues are not conserved, suggesting a distant relationship. GON-14 also contains a putative THAP DNA-binding domain. A rescuing gon-14::GON-14::VENUS reporter is broadly expressed during development and localizes to the nucleus. Strong loss-of-function and predicted null gon-14 alleles have pleiotropic defects, including multivulval (Muv) defects and temperature-sensitive larval arrest. Although the gon-14 Muv defect is not enhanced by synMuv mutations, gon-14 interacts genetically with class B and class C synMuv genes, including lin-35/Rb, let-418/Mi-2beta, and trr-1/TRRAP. The gon-14; synMuv double mutants arrest as larvae when grown under conditions supporting development to adulthood for the respective single mutants. The gon-14 larval arrest is suppressed by loss of mes-2/E(Z), mes-6/ESC, or mes-4, which encodes a SET domain protein. Additionally, gon-14 affects expression of pgl-1 and lag-2, two genes regulated by the synMuv genes. We suggest that gon-14 functions with class B and class C synMuv genes to promote larval growth, in part by antagonizing MES-2,3,6/ESC-E(z) and MES-4. |
巻・号 | 172(2) |
ページ | 915-28 |
公開日 | 2006-2-1 |
DOI | 10.1534/genetics.105.048751 |
PII | genetics.105.048751 |
PMID | 16322520 |
PMC | PMC1383727 |
MeSH | Alleles Amino Acid Motifs Amino Acid Sequence Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / growth & development* Caenorhabditis elegans Proteins / chemistry Caenorhabditis elegans Proteins / classification Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / physiology* Carrier Proteins Cell Cycle Proteins / classification Cell Cycle Proteins / genetics Cell Cycle Proteins / physiology* Cell Division / genetics DNA-Binding Proteins / deficiency DNA-Binding Proteins / genetics DNA-Binding Proteins / physiology* Female Gene Expression Regulation, Developmental / physiology Genes, Helminth* Larva / genetics Larva / growth & development Molecular Sequence Data Nuclear Proteins / deficiency Nuclear Proteins / genetics Nuclear Proteins / physiology Protein Structure, Tertiary Sequence Homology, Amino Acid Transcription Factors / chemistry Transcription Factors / physiology Vulva / growth & development Vulva / physiology* |
IF | 4.015 |
引用数 | 21 |
WOS 分野 | GENETICS & HEREDITY |
リソース情報 | |
線虫 | tm678 |