論文 - 詳細
| RRC ID | 3399 |
|---|---|
| 著者 | Sieburth D, Ch'ng Q, Dybbs M, Tavazoie M, Kennedy S, Wang D, Dupuy D, Rual JF, Hill DE, Vidal M, Ruvkun G, Kaplan JM. |
| タイトル | Systematic analysis of genes required for synapse structure and function. |
| ジャーナル | Nature |
| Abstract |
Chemical synapses are complex structures that mediate rapid intercellular signalling in the nervous system. Proteomic studies suggest that several hundred proteins will be found at synaptic specializations. Here we describe a systematic screen to identify genes required for the function or development of Caenorhabditis elegans neuromuscular junctions. A total of 185 genes were identified in an RNA interference screen for decreased acetylcholine secretion; 132 of these genes had not previously been implicated in synaptic transmission. Functional profiles for these genes were determined by comparing secretion defects observed after RNA interference under a variety of conditions. Hierarchical clustering identified groups of functionally related genes, including those involved in the synaptic vesicle cycle, neuropeptide signalling and responsiveness to phorbol esters. Twenty-four genes encoded proteins that were localized to presynaptic specializations. Loss-of-function mutations in 12 genes caused defects in presynaptic structure. |
| 巻・号 | 436(7050) |
| ページ | 510-7 |
| 公開日 | 2005-7-28 |
| DOI | 10.1038/nature03809 |
| PII | nature03809 |
| PMID | 16049479 |
| MeSH | Aldicarb / pharmacology Animals Caenorhabditis elegans / genetics* Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics* Cluster Analysis Cytoskeleton / metabolism Drug Resistance / genetics Fluorescence Gene Expression Profiling Membrane Proteins / metabolism Microfilament Proteins / metabolism Motor Neurons / metabolism Mutation / genetics Nerve Tissue Proteins / metabolism Neuromuscular Junction / cytology Neuromuscular Junction / genetics Neuromuscular Junction / physiology Neuropeptides / metabolism Phorbol Esters / pharmacology Protein Transport R-SNARE Proteins RNA Interference Synapses / chemistry Synapses / genetics* Synapses / physiology* Synaptic Transmission / genetics* Synaptic Vesicles / metabolism |
| IF | 42.779 |
| 引用数 | 262 |
| WOS 分野 | NEUROSCIENCES |
| リソース情報 | |
| 線虫 | tm292 |