RRC ID 33718
著者 Bauke AC, Sasse S, Matzat T, Klämbt C.
タイトル A transcriptional network controlling glial development in the Drosophila visual system.
ジャーナル Development
Abstract In the nervous system, glial cells need to be specified from a set of progenitor cells. In the developing Drosophila eye, perineurial glia proliferate and differentiate as wrapping glia in response to a neuronal signal conveyed by the FGF receptor pathway. To unravel the underlying transcriptional network we silenced all genes encoding predicted DNA-binding proteins in glial cells using RNAi. Dref and other factors of the TATA box-binding protein-related factor 2 (TRF2) complex were previously predicted to be involved in cellular metabolism and cell growth. Silencing of these genes impaired early glia proliferation and subsequent differentiation. Dref controls proliferation via activation of the Pdm3 transcription factor, whereas glial differentiation is regulated via Dref and the homeodomain protein Cut. Cut expression is controlled independently of Dref by FGF receptor activity. Loss- and gain-of-function studies show that Cut is required for glial differentiation and is sufficient to instruct the formation of membrane protrusions, a hallmark of wrapping glial morphology. Our work discloses a network of transcriptional regulators controlling the progression of a naïve perineurial glia towards the fully differentiated wrapping glia.
巻・号 142(12)
ページ 2184-93
公開日 2015-6-15
DOI 10.1242/dev.119750
PII dev.119750
PMID 26015542
MeSH Animals Cell Proliferation / genetics DNA-Binding Proteins / genetics Drosophila Proteins / biosynthesis Drosophila Proteins / genetics Drosophila Proteins / metabolism Drosophila melanogaster / embryology* Drosophila melanogaster / genetics Enzyme Activation Eye / embryology* Eye / innervation Gene Expression Regulation, Developmental* Gene Regulatory Networks Homeodomain Proteins / biosynthesis Homeodomain Proteins / genetics Homeodomain Proteins / metabolism Neural Stem Cells / cytology* Neurogenesis / genetics* Neuroglia / cytology* Nuclear Proteins / biosynthesis Nuclear Proteins / genetics Nuclear Proteins / metabolism POU Domain Factors / genetics POU Domain Factors / metabolism RNA Interference RNA, Small Interfering Receptors, Fibroblast Growth Factor / metabolism Telomeric Repeat Binding Protein 2 / genetics Transcription Factors / biosynthesis Transcription Factors / genetics Transcription Factors / metabolism Transduction, Genetic
IF 5.611
引用数 20
WOS 分野 DEVELOPMENTAL BIOLOGY
リソース情報
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