RRC ID 33717
著者 Akagawa H, Hara Y, Togane Y, Iwabuchi K, Hiraoka T, Tsujimura H.
タイトル The role of the effector caspases drICE and dcp-1 for cell death and corpse clearance in the developing optic lobe in Drosophila.
ジャーナル Dev Biol
Abstract In the developing Drosophila optic lobe, cell death occurs via apoptosis and in a distinctive spatio-temporal pattern of dying cell clusters. We analyzed the role of effector caspases drICE and dcp-1 in optic lobe cell death and subsequent corpse clearance using mutants. Neurons in many clusters required either drICE or dcp-1 and each one is sufficient. This suggests that drICE and dcp-1 function in cell death redundantly. However, dying neurons in a few clusters strictly required drICE but not dcp-1, but required drICE and dcp-1 when drICE activity was reduced via hypomorphic mutation. In addition, analysis of the mutants suggests an important role of effecter caspases in corpse clearance. In both null and hypomorphic drICE mutants, greater number of TUNEL-positive cells were observed than in wild type, and many TUNEL-positive cells remained until later stages. Lysotracker staining showed that there was a defect in corpse clearance in these mutants. All the results suggested that drICE plays an important role in activating corpse clearance in dying cells, and that an additional function of effector caspases is required for the activation of corpse clearance as well as that for carrying out cell death.
巻・号 404(2)
ページ 61-75
公開日 2015-8-15
DOI 10.1016/j.ydbio.2015.05.013
PII S0012-1606(15)00282-1
PMID 26022392
MeSH Animals Apoptosis / genetics* Caspases / genetics Caspases / metabolism* Drosophila / embryology* Drosophila Proteins / genetics Drosophila Proteins / metabolism* Eye / embryology Eye / innervation In Situ Nick-End Labeling Mutation / genetics Neurons / metabolism Optic Lobe, Nonmammalian / embryology*
IF 2.896
引用数 6
WOS 分野 DEVELOPMENTAL BIOLOGY
リソース情報
ショウジョウバエ HMS00398