| RRC ID |
32551
|
| 著者 |
Lerner DW, McCoy D, Isabella AJ, Mahowald AP, Gerlach GF, Chaudhry TA, Horne-Badovinac S.
|
| タイトル |
A Rab10-dependent mechanism for polarized basement membrane secretion during organ morphogenesis.
|
| ジャーナル |
Dev Cell
|
| Abstract |
Basement membranes (BMs) are specialized extracellular matrices that are essential for epithelial structure and morphogenesis. However, little is known about how BM proteins are delivered to the basal cell surface or how this process is regulated during development. Here, we identify a mechanism for polarized BM secretion in the Drosophila follicle cells. BM proteins are synthesized in a basal endoplasmic reticulum (ER) compartment from localized mRNAs and are then exported through Tango1-positive ER exit sites to basal Golgi clusters. Next, Crag targets Rab10 to structures in the basal cytoplasm, where it restricts protein delivery to the basal surface. These events occur during egg chamber elongation, a morphogenetic process that depends on follicle cell planar polarity and BM remodeling. Significantly, Tango1 and Rab10 are also planar polarized at the basal epithelial surface. We propose that the spatial control of BM production along two tissue axes promotes exocytic efficiency, BM remodeling, and organ morphogenesis.
|
| 巻・号 |
24(2)
|
| ページ |
159-68
|
| 公開日 |
2013-1-28
|
| DOI |
10.1016/j.devcel.2012.12.005
|
| PII |
S1534-5807(12)00578-3
|
| PMID |
23369713
|
| PMC |
PMC3562474
|
| MeSH |
Animals
Aryl Hydrocarbon Receptor Nuclear Translocator / metabolism*
Basement Membrane / metabolism*
Cell Polarity
Collagen Type IV / metabolism
Drosophila / cytology
Drosophila / embryology*
Drosophila / metabolism
Drosophila Proteins / metabolism*
Endoplasmic Reticulum / metabolism
GTP Phosphohydrolases / metabolism
Monomeric GTP-Binding Proteins / metabolism*
Morphogenesis
Organogenesis*
Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase / genetics
Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase / metabolism
rab GTP-Binding Proteins / metabolism
|
| IF |
10.092
|
| 引用数 |
70
|
|
WOS 分野
|
DEVELOPMENTAL BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| ショウジョウバエ |
tj-Gal4 (NP1624
DGRC#104055) |
