論文 - 詳細
| RRC ID | 29121 |
|---|---|
| 著者 | Beatty A, Morton DG, Kemphues K. |
| タイトル | PAR-2, LGL-1 and the CDC-42 GAP CHIN-1 act in distinct pathways to maintain polarity in the C. elegans embryo. |
| ジャーナル | Development |
| Abstract |
In the one-cell C. elegans embryo, polarity is maintained by mutual antagonism between the anterior cortical proteins PAR-3, PKC-3, PAR-6 and CDC-42, and the posterior cortical proteins PAR-2 and LGL-1 on the posterior cortex. The mechanisms by which these proteins interact to maintain polarity are incompletely understood. In this study, we investigate the interplay among PAR-2, LGL-1, myosin, the anterior PAR proteins and CDC-42. We find that PAR-2 and LGL-1 affect cortical myosin accumulation by different mechanisms. LGL-1 does not directly antagonize the accumulation of cortical myosin and instead plays a role in regulating PAR-6 levels. By contrast, PAR-2 likely has separate roles in regulating cortical myosin accumulation and preventing the expansion of the anterior cortical domain. We also provide evidence that asymmetry of active CDC-42 can be maintained independently of LGL-1 and PAR-2 by a redundant pathway that includes the CDC-42 GAP CHIN-1. Finally, we show that, in addition to its primary role in regulating the size of the anterior cortical domain via its binding to PAR-6, CDC-42 has a secondary role in regulating cortical myosin that is not dependent on PAR-6. |
| 巻・号 | 140(9) |
| ページ | 2005-14 |
| 公開日 | 2013-5-1 |
| DOI | 10.1242/dev.088310 |
| PII | dev.088310 |
| PMID | 23536568 |
| PMC | PMC3631973 |
| MeSH | Animals Caenorhabditis elegans / embryology* Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism Cell Polarity* Cytoplasm / genetics Cytoplasm / metabolism Embryo, Nonmammalian / cytology Embryo, Nonmammalian / metabolism Embryonic Development GTP-Binding Proteins / genetics GTP-Binding Proteins / metabolism GTPase-Activating Proteins / genetics GTPase-Activating Proteins / metabolism Gene Expression Regulation, Developmental Guanine Nucleotide Exchange Factors / genetics Guanine Nucleotide Exchange Factors / metabolism Immunohistochemistry Mutation Myosins / metabolism Protein Binding Protein Interaction Mapping Protein Transport RNA Interference |
| IF | 5.611 |
| 引用数 | 22 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY |
| リソース情報 | |
| 線虫 | lgl-1(tm2616) |