| RRC ID |
28851
|
| 著者 |
Clough E, Tedeschi T, Hazelrigg T.
|
| タイトル |
Epigenetic regulation of oogenesis and germ stem cell maintenance by the Drosophila histone methyltransferase Eggless/dSetDB1.
|
| ジャーナル |
Dev Biol
|
| Abstract |
The Drosophila melanogaster histone lysine methyltransferase (HKMT) Eggless (Egg/dSETDB1) catalyzes methylation of Histone H3 lysine 9 (H3K9), a signature of repressive heterochromatin. Our previous studies showed that H3K9 methylation by Egg is required for oogenesis. Here we analyze a set of EMS-induced mutations in the egg gene, identify the molecular lesions of these mutations, and compare the effects on oogenesis of both strong loss-of-function and weak hypomorphic alleles. These studies show that H3K9 methylation by Egg is required for multiple stages of oogenesis. Mosaic expression experiments show that the egg gene is not required intrinsically in the germ cells for their early differentiation, but is required in the germ cells for their survival past stage 5 of oogenesis. egg is also required in germ stem cells for their maintenance, since egg- germ stem cells initially survive but are not maintained as females age. Mosaic analysis also reveals that the early egg chamber budding defects in egg- ovaries are due to an intrinsic requirement for egg in follicle stem cells and their descendents, and that egg plays a non-autonomous role in somatic cells in the germarium to influence the differentiation of early germ cells.
|
| 巻・号 |
388(2)
|
| ページ |
181-91
|
| 公開日 |
2014-4-15
|
| DOI |
10.1016/j.ydbio.2014.01.014
|
| PII |
S0012-1606(14)00042-6
|
| PMID |
24485852
|
| PMC |
PMC4689207
|
| MeSH |
Alleles
Animals
Cell Differentiation
DNA Methylation
Drosophila Proteins / genetics*
Drosophila melanogaster
Epigenesis, Genetic*
Germ Cells / cytology*
Histone-Lysine N-Methyltransferase
Mosaicism
Oogenesis / genetics*
Stem Cells / cytology*
|
| IF |
2.896
|
| 引用数 |
20
|
| リソース情報 |
| ショウジョウバエ |
DGRC#104055 |
