RRC ID |
2862
|
著者 |
Nakatani Y, Nishidate M, Fujita M, Kawakami A, Kudo A.
|
タイトル |
Migration of mesenchymal cell fated to blastema is necessary for fish fin regeneration.
|
ジャーナル |
Dev Growth Differ
|
Abstract |
Urodeles and fish have higher regeneration ability in a variety of tissues and organs than do other vertebrate species including mammals. Though many studies have aimed at identifying the cellular and molecular basis for regeneration, relatively little is known about the detailed cellular behaviors and involved molecular basis. In the present study, a small molecule inhibitor was used to analyzed the role of phosphoinositide 3-kinase (PI3K) signaling during regeneration. We showed that the inhibitor disrupted the formation of blastema including the expression of characteristic genes. The failure of blastema formation was due to the impaired migration of mesenchymal cells to the distal prospective blastema region, although it had a little affect on cell cycle activation in mesenchymal cells. Moreover, we found that the epidermal remodeling including cell proliferation, distal cell migration and Akt phosphorylation was also affected by the inhibitor, implying a possible involvement of epidermis for proper formation of blastema. From these data, we propose a model in which distinct signals that direct the cell cycle activation, mesenchymal cell migration and epidermal remodeling coordinate together to accomplish the correct blastema formation and regeneration.
|
巻・号 |
50(2)
|
ページ |
71-83
|
公開日 |
2008-2-1
|
DOI |
10.1111/j.1440-169X.2007.00977.x
|
PII |
DGD977
|
PMID |
18042247
|
MeSH |
Androstadienes / pharmacology
Animals
Animals, Genetically Modified
Blotting, Western
Cell Movement*
Chromones / pharmacology
Enzyme Inhibitors / pharmacology
Immunochemistry
In Situ Hybridization
Mesenchymal Stem Cells / cytology*
Microscopy, Confocal
Morpholines / pharmacology
Neovascularization, Physiologic
Oryzias / anatomy & histology
Oryzias / physiology*
Phosphatidylinositol 3-Kinases / metabolism
Phosphoinositide-3 Kinase Inhibitors
Regeneration*
Signal Transduction
Wortmannin
|
IF |
1.723
|
引用数 |
17
|
WOS 分野
|
DEVELOPMENTAL BIOLOGY
CELL BIOLOGY
|
リソース情報 |
メダカ |
OK-Cab (MT830)
Qurt(MT828) |