| RRC ID |
2831
|
| 著者 |
Bubenshchikova E, Kaftanovskaya E, Motosugi N, Fujimoto T, Arai K, Kinoshita M, Hashimoto H, Ozato K, Wakamatsu Y.
|
| タイトル |
Diploidized eggs reprogram adult somatic cell nuclei to pluripotency in nuclear transfer in medaka fish (Oryzias latipes).
|
| ジャーナル |
Dev Growth Differ
|
| Abstract |
Reprogramming of adult somatic cell nuclei to pluripotency has been unsuccessful in non-mammalian animals, primarily because of chromosomal aberrations in nuclear transplants, which are considered to be caused by asynchrony between the cell cycles of the recipient egg and donor nucleus. In order to normalize the chromosomal status, we used diploidized eggs by retention of second polar body release, instead of enucleated eggs, as recipients in nuclear transfer of primary culture cells from the caudal fin of adult green fluorescent protein gene (GFP) transgenic medaka fish (Oryzias latipes). We found that 2.7% of the reconstructed embryos grew into adults that expressed GFP in various tissues in the same pattern as in the donor fish. Moreover, these fish were diploid, fertile and capable of passing the marker gene to the next generation in Mendelian fashion. We hesitate to call these fish 'clones' because we used non-enucleated eggs as recipients; in effect, they may be chimeras consisting of cells derived from diploid recipient nuclei and donor nuclei. In either case, fish adult somatic cell nuclei were reprogrammed to pluripotency and differentiated into a variety of cell types including germ cells via the use of diploidized recipient eggs.
|
| 巻・号 |
49(9)
|
| ページ |
699-709
|
| 公開日 |
2007-12-1
|
| DOI |
10.1111/j.1440-169X.2007.00963.x
|
| PII |
DGD963
|
| PMID |
17868280
|
| MeSH |
Animals
Cell Nucleus / genetics*
Cell Nucleus / metabolism
Diploidy*
Embryo, Nonmammalian / cytology
Embryo, Nonmammalian / metabolism
Flow Cytometry
Green Fluorescent Proteins / genetics
Green Fluorescent Proteins / metabolism
Microscopy, Fluorescence
Models, Biological
Nuclear Transfer Techniques
Oryzias / genetics*
Ovum / cytology
Ovum / metabolism*
Ploidies
Pluripotent Stem Cells / cytology
Pluripotent Stem Cells / metabolism
|
| IF |
1.723
|
| 引用数 |
19
|
|
WOS 分野
|
DEVELOPMENTAL BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| メダカ |
Tg(ß-actin-GFP) (TG129) |
