RRC ID 221
著者 Kuwahara Y, Shimada A, Mitani H, Shima A.
タイトル Gamma-ray exposure accelerates spermatogenesis of medaka fish, Oryzias latipes.
ジャーナル Mol Reprod Dev
Abstract To examine the spermatogenesis (and spermiogenesis) cell population kinetics after gamma-irradiation, the frequency and fate of BrdU-labeled pre-meiotic spermatogenic cells (spermatogonia and pre-leptotene spermatocytes) and spermatogonial stem cells (SSCs) of the medaka fish (Oryzias latipes) were examined immunohistochemically and by BrdU-labeling. After 4.75 Gy of gamma-irradiation, a statistically significant decrease in the frequency of BrdU-labeled cells was detected in the SSCs, but not in pre-meiotic spermatogenic cells. The time necessary for differentiation of surviving pre-meiotic spermatogenic cells without delay of germ cell development was shortened. More than 90% of surviving pre-meiotic spermatogenic cells differentiated into haploid cells within 5 days after irradiation, followed by a temporal spermatozoa exhaust in the testis. Next, spermatogenesis began in the surviving SSCs. However, the outcome was abnormal spermatozoa, indicating that accelerated maturation process led to morphological abnormalities. Moreover, 35% of the morphologically normal spermatozoa were dead at day 6. Based on these results, we suggest a reset system; after irradiation most surviving spermatogenic cells, except for the SSCs, are prematurely eliminated from the testis by spermatogenesis (and spermiogenesis) acceleration, and subsequent spermatogenesis begins with the surviving SSCs, a possible safeguard against male germ cell mutagenesis.
巻・号 65(2)
ページ 204-11
公開日 2003-6-1
DOI 10.1002/mrd.10261
PMID 12704732
MeSH Animals Dose-Response Relationship, Radiation Gamma Rays* Male Oryzias / physiology* Spermatogenesis / radiation effects* Testis / radiation effects
IF 2.823
引用数 12
WOS 分野 REPRODUCTIVE BIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY DEVELOPMENTAL BIOLOGY CELL BIOLOGY
リソース情報
メダカ HNI-II (IB176)