RRC ID 21939
著者 Buchon N, Broderick NA, Chakrabarti S, Lemaitre B.
タイトル Invasive and indigenous microbiota impact intestinal stem cell activity through multiple pathways in Drosophila.
ジャーナル Genes Dev
Abstract Gut homeostasis is controlled by both immune and developmental mechanisms, and its disruption can lead to inflammatory disorders or cancerous lesions of the intestine. While the impact of bacteria on the mucosal immune system is beginning to be precisely understood, little is known about the effects of bacteria on gut epithelium renewal. Here, we addressed how both infectious and indigenous bacteria modulate stem cell activity in Drosophila. We show that the increased epithelium renewal observed upon some bacterial infections is a consequence of the oxidative burst, a major defense of the Drosophila gut. Additionally, we provide evidence that the JAK-STAT (Janus kinase-signal transducers and activators of transcription) and JNK (c-Jun NH(2) terminal kinase) pathways are both required for bacteria-induced stem cell proliferation. Similarly, we demonstrate that indigenous gut microbiota activate the same, albeit reduced, program at basal levels. Altered control of gut microbiota in immune-deficient or aged flies correlates with increased epithelium renewal. Finally, we show that epithelium renewal is an essential component of Drosophila defense against oral bacterial infection. Altogether, these results indicate that gut homeostasis is achieved by a complex interregulation of the immune response, gut microbiota, and stem cell activity.
巻・号 23(19)
ページ 2333-44
公開日 2009-10-1
DOI 10.1101/gad.1827009
PII 23/19/2333
PMID 19797770
PMC PMC2758745
MeSH Animals Cell Proliferation Drosophila Proteins / metabolism Drosophila melanogaster / cytology* Drosophila melanogaster / microbiology* Epithelium / microbiology Gene Expression Regulation, Developmental Intestines / cytology Intestines / immunology Intestines / microbiology Janus Kinases / metabolism Pectobacterium carotovorum / physiology* Pseudomonas / physiology* Respiratory Burst STAT Transcription Factors / metabolism Stem Cells / cytology Stem Cells / microbiology Transcription Factors / metabolism
IF 9.527
引用数 411
WOS 分野 DEVELOPMENTAL BIOLOGY GENETICS & HEREDITY CELL BIOLOGY
リソース情報
ショウジョウバエ 12297R-1 STAT (R2) 2275R-1 basket (R1) 2190R-1