論文 - 詳細
| RRC ID | 21904 |
|---|---|
| 著者 | Sadaf S, Birman S, Hasan G. |
| タイトル | Synaptic activity in serotonergic neurons is required for air-puff stimulated flight in Drosophila melanogaster. |
| ジャーナル | PLoS One |
| Abstract |
BACKGROUND:Flight is an integral component of many complex behavioral patterns in insects. The giant fiber circuit has been well studied in several insects including Drosophila. However, components of the insect flight circuit that respond to an air-puff stimulus and comprise the flight central pattern generator are poorly defined. Aminergic neurons have been implicated in locust, moth and Drosophila flight. Here we have investigated the requirement of neuronal activity in serotonergic neurons, during development and in adults, on air-puff induced flight in Drosophila. METHODOLOGY/PRINCIPAL FINDINGS:To target serotonergic neurons specifically, a Drosophila strain that contains regulatory regions from the TRH (Tryptophan Hydroxylase) gene linked to the yeast transcription factor GAL4 was used. By blocking synaptic transmission from serotonergic neurons with a tetanus toxin transgene or by hyperpolarisation with Kir2.1, close to 50% adults became flightless. Temporal expression of a temperature sensitive Dynamin mutant transgene (Shi(ts)) suggests that synaptic function in serotonergic neurons is required both during development and in adults. Depletion of IP(3)R in serotonergic neurons via RNAi did not affect flight. Interestingly, at all stages a partial requirement for synaptic activity in serotonergic neurons was observed. The status of serotonergic neurons was investigated in the central nervous system of larvae and adults expressing tetanus toxin. A small but significant reduction was observed in serotonergic cell number in adult second thoracic segments from flightless tetanus toxin expressing animals. CONCLUSIONS:These studies show that loss of synaptic activity in serotonergic neurons causes a flight deficit. The temporal focus of the flight deficit is during pupal development and in adults. The cause of the flight deficit is likely to be loss of neurons and reduced synaptic function. Based on the partial phenotypes, serotonergic neurons appear to be modulatory, rather than an intrinsic part of the flight circuit. |
| 巻・号 | 7(9) |
| ページ | e46405 |
| 公開日 | 2012-9-27 |
| DOI | 10.1371/journal.pone.0046405 |
| PII | PONE-D-12-12918 |
| PMID | 23029511 |
| PMC | PMC3459902 |
| MeSH | Animals Cell Count Central Nervous System / cytology Central Nervous System / metabolism DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism Drosophila Proteins / genetics Drosophila Proteins / metabolism Drosophila melanogaster / physiology* Dynamins / genetics Dynamins / metabolism Flight, Animal / physiology* Gene Expression Regulation, Developmental Larva / physiology Potassium Channels, Inwardly Rectifying / genetics Potassium Channels, Inwardly Rectifying / metabolism Pupa / physiology Saccharomyces cerevisiae Proteins / genetics Saccharomyces cerevisiae Proteins / metabolism Serotonergic Neurons / cytology Serotonergic Neurons / metabolism* Synaptic Transmission / physiology* Tetanus Toxin / genetics Tetanus Toxin / metabolism Transcription Factors / genetics Transcription Factors / metabolism Transgenes Tryptophan Hydroxylase / genetics Tryptophan Hydroxylase / metabolism |
| IF | 2.74 |
| 引用数 | 11 |
| WOS 分野 | NEUROSCIENCES |
| リソース情報 | |
| ショウジョウバエ | 1063R-1 1063R-2 |