RRC ID 21893
著者 Agrawal N, Venkiteswaran G, Sadaf S, Padmanabhan N, Banerjee S, Hasan G.
タイトル Inositol 1,4,5-trisphosphate receptor and dSTIM function in Drosophila insulin-producing neurons regulates systemic intracellular calcium homeostasis and flight.
ジャーナル J Neurosci
Abstract Calcium (Ca(2+)) signaling is known to regulate the development, maintenance and modulation of activity in neuronal circuits that underlie organismal behavior. In Drosophila, intracellular Ca(2+) signaling by the inositol 1,4,5-trisphosphate receptor and the store-operated channel (dOrai) regulates the formation and function of neuronal circuits that control flight. Here, we show that restoring InsP(3)R activity in insulin-producing neurons of flightless InsP(3)R mutants (itpr) during pupal development can rescue systemic flight ability. Expression of the store operated Ca(2+) entry (SOCE) regulator dSTIM in insulin-producing neurons also suppresses compromised flight ability of InsP(3)R mutants suggesting that SOCE can compensate for impaired InsP(3)R function. Despite restricted expression of wild-type InsP(3)R and dSTIM in insulin-producing neurons, a global restoration of SOCE and store Ca(2+) is observed in primary neuronal cultures from the itpr mutant. These results suggest that restoring InsP(3)R-mediated Ca(2+) release and SOCE in a limited subset of neuromodulatory cells can influence systemic behaviors such as flight by regulating intracellular Ca(2+) homeostasis in a large population of neurons through a non-cell-autonomous mechanism.
巻・号 30(4)
ページ 1301-13
公開日 2010-1-27
DOI 10.1523/JNEUROSCI.3668-09.2010
PII 30/4/1301
PMID 20107057
PMC PMC6633787
MeSH Animals Calcium / metabolism Calcium Signaling / physiology* Cell Membrane / genetics Cell Membrane / metabolism Cells, Cultured Central Nervous System / cytology Central Nervous System / growth & development Central Nervous System / metabolism* Drosophila Drosophila Proteins / genetics Drosophila Proteins / metabolism* Flight, Animal / physiology Homeostasis / physiology Inositol 1,4,5-Trisphosphate Receptors / genetics Inositol 1,4,5-Trisphosphate Receptors / metabolism* Insulin / metabolism* Insulin Secretion Intracellular Fluid / metabolism Membrane Proteins / genetics Membrane Proteins / metabolism* Mutation / genetics Neural Pathways / cytology Neural Pathways / growth & development Neural Pathways / metabolism Neurons / cytology Neurons / metabolism* Pupa / genetics Pupa / growth & development Pupa / metabolism Stromal Interaction Molecule 1
IF 5.674
引用数 32
WOS 分野 NEUROSCIENCES
リソース情報
ショウジョウバエ 1063R-2