RRC ID |
21893
|
著者 |
Agrawal N, Venkiteswaran G, Sadaf S, Padmanabhan N, Banerjee S, Hasan G.
|
タイトル |
Inositol 1,4,5-trisphosphate receptor and dSTIM function in Drosophila insulin-producing neurons regulates systemic intracellular calcium homeostasis and flight.
|
ジャーナル |
J Neurosci
|
Abstract |
Calcium (Ca(2+)) signaling is known to regulate the development, maintenance and modulation of activity in neuronal circuits that underlie organismal behavior. In Drosophila, intracellular Ca(2+) signaling by the inositol 1,4,5-trisphosphate receptor and the store-operated channel (dOrai) regulates the formation and function of neuronal circuits that control flight. Here, we show that restoring InsP(3)R activity in insulin-producing neurons of flightless InsP(3)R mutants (itpr) during pupal development can rescue systemic flight ability. Expression of the store operated Ca(2+) entry (SOCE) regulator dSTIM in insulin-producing neurons also suppresses compromised flight ability of InsP(3)R mutants suggesting that SOCE can compensate for impaired InsP(3)R function. Despite restricted expression of wild-type InsP(3)R and dSTIM in insulin-producing neurons, a global restoration of SOCE and store Ca(2+) is observed in primary neuronal cultures from the itpr mutant. These results suggest that restoring InsP(3)R-mediated Ca(2+) release and SOCE in a limited subset of neuromodulatory cells can influence systemic behaviors such as flight by regulating intracellular Ca(2+) homeostasis in a large population of neurons through a non-cell-autonomous mechanism.
|
巻・号 |
30(4)
|
ページ |
1301-13
|
公開日 |
2010-1-27
|
DOI |
10.1523/JNEUROSCI.3668-09.2010
|
PII |
30/4/1301
|
PMID |
20107057
|
PMC |
PMC6633787
|
MeSH |
Animals
Calcium / metabolism
Calcium Signaling / physiology*
Cell Membrane / genetics
Cell Membrane / metabolism
Cells, Cultured
Central Nervous System / cytology
Central Nervous System / growth & development
Central Nervous System / metabolism*
Drosophila
Drosophila Proteins / genetics
Drosophila Proteins / metabolism*
Flight, Animal / physiology
Homeostasis / physiology
Inositol 1,4,5-Trisphosphate Receptors / genetics
Inositol 1,4,5-Trisphosphate Receptors / metabolism*
Insulin / metabolism*
Insulin Secretion
Intracellular Fluid / metabolism
Membrane Proteins / genetics
Membrane Proteins / metabolism*
Mutation / genetics
Neural Pathways / cytology
Neural Pathways / growth & development
Neural Pathways / metabolism
Neurons / cytology
Neurons / metabolism*
Pupa / genetics
Pupa / growth & development
Pupa / metabolism
Stromal Interaction Molecule 1
|
IF |
5.674
|
引用数 |
32
|
WOS 分野
|
NEUROSCIENCES
|
リソース情報 |
ショウジョウバエ |
1063R-2 |