論文 - 詳細
| RRC ID | 21883 |
|---|---|
| 著者 | Jafari S, Alkhori L, Schleiffer A, Brochtrup A, Hummel T, Alenius M. |
| タイトル | Combinatorial activation and repression by seven transcription factors specify Drosophila odorant receptor expression. |
| ジャーナル | PLoS Biol |
| Abstract |
The mechanism that specifies olfactory sensory neurons to express only one odorant receptor (OR) from a large repertoire is critical for odor discrimination but poorly understood. Here, we describe the first comprehensive analysis of OR expression regulation in Drosophila. A systematic, RNAi-mediated knock down of most of the predicted transcription factors identified an essential function of acj6, E93, Fer1, onecut, sim, xbp1, and zf30c in the regulation of more than 30 ORs. These regulatory factors are differentially expressed in antennal sensory neuron classes and specifically required for the adult expression of ORs. A systematic analysis reveals not only that combinations of these seven factors are necessary for receptor gene expression but also a prominent role for transcriptional repression in preventing ectopic receptor expression. Such regulation is supported by bioinformatics and OR promoter analyses, which uncovered a common promoter structure with distal repressive and proximal activating regions. Thus, our data provide insight into how combinatorial activation and repression can allow a small number of transcription factors to specify a large repertoire of neuron classes in the olfactory system. |
| 巻・号 | 10(3) |
| ページ | e1001280 |
| 公開日 | 2012-3-13 |
| DOI | 10.1371/journal.pbio.1001280 |
| PII | PBIOLOGY-D-11-05202 |
| PMID | 22427741 |
| PMC | PMC3302810 |
| MeSH | Animals Arthropod Antennae / cytology Arthropod Antennae / metabolism Arthropod Antennae / physiology Basic Helix-Loop-Helix Transcription Factors / genetics Basic Helix-Loop-Helix Transcription Factors / metabolism Binding Sites Computational Biology DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism Drosophila / genetics* Drosophila / metabolism Drosophila / physiology Drosophila Proteins / genetics Drosophila Proteins / metabolism* Gene Expression Regulation, Developmental* Gene Knockdown Techniques Genes, Insect Mutagenesis, Insertional Nerve Tissue Proteins / genetics Nerve Tissue Proteins / metabolism Neurons / metabolism Nuclear Proteins / genetics Nuclear Proteins / metabolism Nucleotide Motifs POU Domain Factors / genetics POU Domain Factors / metabolism Promoter Regions, Genetic RNA Interference Receptors, Odorant / genetics Receptors, Odorant / metabolism* Sensory Receptor Cells / cytology Sensory Receptor Cells / metabolism Sensory Receptor Cells / physiology Species Specificity Transcription Factors / genetics Transcription Factors / metabolism* Transcriptional Activation |
| IF | 7.076 |
| 引用数 | 45 |
| WOS 分野 | BIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| ショウジョウバエ | 18389R-1 10066R-4 1922R-2 3998R-1 |