| RRC ID |
21867
|
| 著者 |
Boulanger A, Farge M, Ramanoudjame C, Wharton K, Dura JM.
|
| タイトル |
Drosophila motor neuron retraction during metamorphosis is mediated by inputs from TGF-β/BMP signaling and orphan nuclear receptors.
|
| ジャーナル |
PLoS One
|
| Abstract |
Larval motor neurons remodel during Drosophila neuro-muscular junction dismantling at metamorphosis. In this study, we describe the motor neuron retraction as opposed to degeneration based on the early disappearance of β-Spectrin and the continuing presence of Tubulin. By blocking cell dynamics with a dominant-negative form of Dynamin, we show that phagocytes have a key role in this process. Importantly, we show the presence of peripheral glial cells close to the neuro-muscular junction that retracts before the motor neuron. We show also that in muscle, expression of EcR-B1 encoding the steroid hormone receptor required for postsynaptic dismantling, is under the control of the ftz-f1/Hr39 orphan nuclear receptor pathway but not the TGF-β signaling pathway. In the motor neuron, activation of EcR-B1 expression by the two parallel pathways (TGF-β signaling and nuclear receptor) triggers axon retraction. We propose that a signal from a TGF-β family ligand is produced by the dismantling muscle (postsynapse compartment) and received by the motor neuron (presynaptic compartment) resulting in motor neuron retraction. The requirement of the two pathways in the motor neuron provides a molecular explanation for the instructive role of the postsynapse degradation on motor neuron retraction. This mechanism insures the temporality of the two processes and prevents motor neuron pruning before postsynaptic degradation.
|
| 巻・号 |
7(7)
|
| ページ |
e40255
|
| 公開日 |
2012-7-5
|
| DOI |
10.1371/journal.pone.0040255
|
| PII |
PONE-D-12-01465
|
| PMID |
22792255
|
| PMC |
PMC3390346
|
| MeSH |
Animals
Bone Morphogenetic Proteins / metabolism*
Bone Morphogenetic Proteins / physiology
Cell Adhesion Molecules / metabolism
Drosophila Proteins / metabolism*
Drosophila Proteins / physiology
Drosophila melanogaster / cytology*
Drosophila melanogaster / growth & development
Gene Expression Regulation, Developmental
Larva / cytology
Larva / growth & development
Metamorphosis, Biological*
Microtubule-Associated Proteins / metabolism*
Microtubule-Associated Proteins / physiology
Motor Neurons / cytology
Motor Neurons / metabolism*
Motor Neurons / physiology
Neuroglia / physiology
Neuromuscular Junction / growth & development
Neuromuscular Junction / metabolism
Neuromuscular Junction / physiology
Orphan Nuclear Receptors / metabolism*
Phagocytosis
Receptors, Steroid / genetics
Receptors, Steroid / metabolism
Signal Transduction
Spectrin / metabolism
Transforming Growth Factor beta / metabolism*
Transforming Growth Factor beta / physiology
Tubulin / metabolism
|
| IF |
2.74
|
| 引用数 |
16
|
|
WOS 分野
|
NEUROSCIENCES
|
| リソース情報 |
| ショウジョウバエ |
UAS-baboRNAi (on X)
12399R-1
12399R-2
7904R-2 |
