RRC ID 1781
著者 Kobayashi M, Michaut L, Ino A, Honjo K, Nakajima T, Maruyama Y, Mochizuki H, Ando M, Ghangrekar I, Takahashi K, Saigo K, Ueda R, Gehring WJ, Furukubo-Tokunaga K.
タイトル Differential microarray analysis of Drosophila mushroom body transcripts using chemical ablation.
ジャーナル Proc Natl Acad Sci U S A
Abstract Mushroom bodies (MBs) are the centers for olfactory associative learning and elementary cognitive functions in the Drosophila brain. As a way to systematically elucidate genes preferentially expressed in MBs, we have analyzed genome-wide alterations in transcript profiles associated with MB ablation by hydroxyurea. We selected 100 genes based on microarray data and examined their expression patterns in the brain by in situ hybridization. Seventy genes were found to be expressed in the posterodorsal cortex, which harbors the MB cell bodies. These genes encode proteins of diverse functions, including transcription, signaling, cell adhesion, channels, and transporters. Moreover, we have examined developmental functions of 40 of the microarray-identified genes by transgenic RNA interference; 8 genes were found to cause mild-to-strong MB defects when suppressed with a MB-Gal4 driver. These results provide important information not only on the repertoire of genes that control MB development but also on the repertoire of neural factors that may have important physiological functions in MB plasticity.
巻・号 103(39)
ページ 14417-22
公開日 2006-9-26
DOI 10.1073/pnas.0606571103
PII 0606571103
PMID 16971484
PMC PMC1599978
MeSH Animals Brain / cytology Brain / drug effects Drosophila melanogaster / genetics* Female Gene Expression Regulation, Developmental Genes, Insect / genetics Hydroxyurea / pharmacology* Microarray Analysis / methods* Mushroom Bodies / abnormalities Mushroom Bodies / cytology Mushroom Bodies / drug effects* RNA Interference RNA, Messenger / genetics*
IF 9.412
引用数 23
WOS 分野 NEUROSCIENCES
リソース情報
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